NDLI: Enhanced cold stress tolerance of transgenic Dendrocalamus latiflorus Munro (Ma bamboo) plants expressing a bacterial CodA gene

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Enhanced cold stress tolerance of transgenic Dendrocalamus latiflorus Munro (Ma bamboo) plants expressing a bacterial CodA gene

Content Provider	Springer Nature Link
Author	Qiao, Guirong Yang, Huiqing Zhang, Ling Han, Xiaojiao Liu, Mingying Jiang, Jing Jiang, Yancheng Zhuo, Renying
Copyright Year	2014
Abstract	Ma bamboo (Dendrocalamus latiflorus Munro) is a widespread culm and shoot-producing species in southern China. However, low temperatures reduce Ma bamboo shoot production and delay its development. In an attempt to enhance its cold-tolerance, a bacterial CodA gene encoding choline oxidase was introduced into Ma bamboo by Agrobacterium-mediated transformation, an approach that had not been previously utilized in bamboo. PCR and Southern blot analyses confirmed that CodA had integrated into the Ma bamboo genome. RT-PCR results showed that expression of CodA driven by the Arabidopsis Rd29A promoter was induced by cold stress in the transgenic bamboo lines. Following treatment at 4°C for 24 h, the content of glycine betaine (GB) increased to 83% and 140% in control plants (wild type (WT)) and CodA transgenic Ma bamboo plants, respectively. Superoxide dismutase, peroxidase, and catalase activities increased in both transgenic and WT plants. However, increases in these enzymes activities were much greater in the transgenic lines than in the WT plants under cold stress. The accumulation of malondialdehyde and electrolyte leakage (REL) in CodA transgenic Ma bamboo plants was less than that in control plants. Collectively, these results suggest that increased cold-tolerance induced by accumulation of GB in vivo was associated with the enhancement of antioxidant enzyme activities, which led to reduced accumulation of reactive oxygen species and stabilization of membrane integrity against extreme temperatures in transgenic plants.
Starting Page	385
Ending Page	391
Page Count	7
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	50
Issue Number	4
e-ISSN	14752689
Language	English
Publisher	Springer US
Publisher Date	2014-03-08
Publisher Place	Boston
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dendrocalamus latiflorus Munro Gene transformation CodA gene Cold stress Plant Sciences Cell Biology Developmental Biology Plant Breeding/Biotechnology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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