NDLI: Development of an efficient tissue culture protocol for callus formation and plant regeneration of wetland species Juncus effusus L.

Content Provider	Springer Nature Link
Author	Xu, L. Najeeb, U. Raziuddin, R. Shen, W. Q. Shou, J. Y. Tang, G. X. Zhou, W. J.
Copyright Year	2009
Abstract	Wetland species mat rush (Juncus effusus L.) is an important economic plant, but no information is available regarding plant regeneration, callus induction, and its proliferation from in vitro seed grown plantlets. The present study investigates the effects of growth regulator combinations and medium innovation on tissue culture system of five mat rush varieties. Addition of N6-benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) in Murashige and Skoog (MS) medium showed significantly positive effect on callus proliferation, plant regeneration, and its multiplication compared to the medium devoid of BA. The highest callus induction frequency (80.95%, 90.48%, 75.40%, 70.83%, and 83.33%) was observed in MS medium containing 0.5 mg L$^{−1}$ (2.2 μM) BA in Yinlin-1, Nonglin-4, Gangshan, Taicao, and Taiwan green, respectively. Various growth regulator combinations with successive subculture (medium replacement) were found essential to develop organogenic calluses and to regenerate shoots. The combination of 0.1 mg L$^{−1}$ BA (0.4 μM) and 2 mg L$^{−1}$ 2,4-D (9.0 μM) in MS medium was found best for callus proliferation for all the varieties under trial. The plant regeneration required two steps involving successive medium replacements as well as optimal hormonal balances. Successful plant regeneration (over 70%) was observed only by transferring the organogenic callus from regeneration medium I [MS medium containing 0.5 mg L$^{−1}$ BA (2. μM) and 1.0 mg L$^{−1}$ kinetin (KT; 4.6 μM)] to the regeneration medium II [MS medium containing 0.5 mg L$^{−1}$ BA (2.2 μM), 1.0 mg L$^{−1}$ KT (4.6 μM) and 3.0 mg L$^{−1}$ indoleacetic acid (IAA; 17.1 μM)]. Our results confirmed the importance of the ratio of auxin (IAA) to cytokinin (BA and KT) in the manipulation of shoot regeneration in J. effusus L. The maximum plant survival frequency and multiplication rates (90.97% and 5.40 and 94.23% and 8.25) were recorded in the presence of 0.5 mg L$^{−1}$ BA (2.2 μM) in the 1/2 MS multiplication medium for the varieties of Nonglin-4 and Taicao, respectively. About 100% survival rate was also observed for all the varieties in soil conditions. The efficient plant regeneration system developed here will be helpful for rapid micropropagation and further genetic improvement in J. effusus L.
Starting Page	610
Ending Page	618
Page Count	9
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	45
Issue Number	5
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-07-08
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Callus induction Juncus effusus L. Medium innovation N6-benzyladenine Plant regeneration Plant Genetics & Genomics Plant Breeding/Biotechnology Developmental Biology Cell Biology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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