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  1. In Vitro Cellular & Developmental Biology - Plant
  2. In Vitro Cellular & Developmental Biology - Plant : Volume 39
  3. In Vitro Cellular & Developmental Biology - Plant : Volume 39, Issue 6, November 2003
  4. Transformation of sugar beet cell suspension cultures
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In Vitro Cellular & Developmental Biology - Plant : Volume 53
In Vitro Cellular & Developmental Biology - Plant : Volume 52
In Vitro Cellular & Developmental Biology - Plant : Volume 51
In Vitro Cellular & Developmental Biology - Plant : Volume 50
In Vitro Cellular & Developmental Biology - Plant : Volume 49
In Vitro Cellular & Developmental Biology - Plant : Volume 48
In Vitro Cellular & Developmental Biology - Plant : Volume 47
In Vitro Cellular & Developmental Biology - Plant : Volume 46
In Vitro Cellular & Developmental Biology - Plant : Volume 45
In Vitro Cellular & Developmental Biology - Plant : Volume 44
In Vitro Cellular & Developmental Biology - Plant : Volume 43
In Vitro Cellular & Developmental Biology - Plant : Volume 42
In Vitro Cellular & Developmental Biology - Plant : Volume 41
In Vitro Cellular & Developmental Biology - Plant : Volume 40
In Vitro Cellular & Developmental Biology - Plant : Volume 39
In Vitro Cellular & Developmental Biology - Plant : Volume 39, Issue 6, November 2003
Analyzing somaclonal variation in micropropagated bananas (Musa spp.)
Novel approaches for regulating gas supply to plant systems in vitro: Application and benefits of artificial gas carriers
Citrus tristeza virus (CTV) resistance in transgenic citrus based on virus challenge of protoplasts
Transformation of sugar beet cell suspension cultures
Tissue culture and Agrobacterium-mediated transformation of hemp (Cannabis sativa L.)
Electroporation of embryogenic protoplasts of sweet orange (Citrus sinensis (L.) Osbeck) and regeneration of transformed plants
Desiccation of plant tissues post-Agrobacterium infection enhances T-DNA delivery and increases stable transformation efficiency in wheat
In vitro regeneration of Echinacea purpurea L.: Enhancement of somatic embryogenesis by indolebutyric acid and dark pre-incubation
Plant regeneration from encapsulated somatic embryos of pedunculate oak (Quercus robur L.)
Axillary bud development of passionfruit as affected by ethylene precursor and inhibitors
Effects of proliferation, maturation, and desiccation methods on conversion of soybean somatic embryos
Somatic embryogenesis from immature zygotic embryos of annatto (Bixa orellana L.)
Highly-efficient somatic embryogenesis from cell suspension cultures of phalaenopsis orchids by adjusting carbohydrate sources
Comparison of the influence of different elicitors on hyoscyamine and scopolamine content in hairy root cultures of Brugmansia candida
Carbohydrate, metabolic, and osmotic changes in scaled-up liquid cultures of Narcissus leaves
Physiology of effects of temporary immersion bioreactors on micropropagated pineapple plantlets
Characterization of ploidy levels of wheat microspore-derived plants using laser flow cytometry
Genetically modified organisms-transgenesis in plants
In Vitro Cellular & Developmental Biology - Plant : Volume 39, Issue 5, September 2003
In Vitro Cellular & Developmental Biology - Plant : Volume 39, Issue 4, July 2003
In Vitro Cellular & Developmental Biology - Plant : Volume 39, Issue 3, May 2003
In Vitro Cellular & Developmental Biology - Plant : Volume 39, Issue 2, March 2003
In Vitro Cellular & Developmental Biology - Plant : Volume 39, Issue 1, January 2003
In Vitro Cellular & Developmental Biology - Plant : Volume 38
In Vitro Cellular & Developmental Biology - Plant : Volume 37
In Vitro Cellular & Developmental Biology - Plant : Volume 36
In Vitro Cellular & Developmental Biology - Plant : Volume 35
In Vitro Cellular & Developmental Biology - Plant : Volume 34
In Vitro Cellular & Developmental Biology - Plant : Volume 33

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Transformation of sugar beet cell suspension cultures

Content Provider Springer Nature Link
Author Ivic, Snezana D. Smigocki, Ann C.
Copyright Year 2003
Abstract A sugar beet transformation method was developed using particle bombardment of short-term suspension cultures of a breeding line FC607. Highly embryogenic suspension cultures derived from leaf callus were bombarded with the uidA (gusA) reporter gene under the control of either the osmotin or proteinase inhibitor II gene promoter, and the npt II selectable marker gene. Transient uidA expression was visualized as 500–4000 blue units per 200 mg of bombarded cells 2 d after bombardment. Stably-transformed calluses were recovered on both kanamycin and paromomycin media. The greatest number of GUS (+) calluses was obtained when 50 or 100 mgl$^{−1}$ of kanamycin was applied 2 d after transformation for 3–5 wk, followed by either no selection or reduced levels of the antibiotic. PCR analyses of the GUS (+) callus lines revealed the expected size fragment for uidA and npt II genes. Stable incorporation of the uidA gene into the genome was confirmed by Southern blot analyses. Several transformed embryos were detected by histochemical β-glucuronidase (GUS) staining.
Starting Page 573
Ending Page 577
Page Count 5
File Format PDF
ISSN 10545476
Journal In Vitro Cellular & Developmental Biology - Plant
Volume Number 39
Issue Number 6
e-ISSN 14752689
Language English
Publisher Springer-Verlag
Publisher Date 2003-01-01
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Beta vulgaris biolistic embryogenesis npt II particle bombardment uidA Plant Biochemistry Cell Biology Developmental Biology Plant Genetics & Genomics Plant Physiology
Content Type Text
Resource Type Article
Subject Cell Biology Developmental Biology Plant Science Biotechnology
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