NDLI: Culture of isolated zygotic embryos of Pinus radiata D. Don. Part I: Factors influencing in vitro germination and growth of isolated embryos

Content Provider	Springer Nature Link
Author	Lin, Xueqin Leung, David W. M.
Copyright Year	2002
Abstract	Factors influeneing the conversion of isolated mature zygotic embryos of Pinus radiata D. Don into plantlets were investigated. Nutritional factors were critical to this conversion. The optimum medium strength was half-strength medium consisting of modified Quoirin and Le Poivre salts (von Arnold and Eriksson, 1981) and Schenk and Hildebrandt (1972) vitamins. Sucrose (3%), glucose (2–3%) and fructose (2–5%) could serve as carbon sources for this conversion. In general, a few significant benefits were found with the addition of organic nitrogen sources tested on the development of isolated embryos into plantlets. Nearly all plant growth regulators tested were not beneficial for this conversion, and some of them had a negative effect. Only gibberellic acid (GA$_{3}$ at 0.58 μM) seemed to stimulate embryos to germinate a little bit earlier in comparison with the control. Submerging the cotyledons of the isolated embryo into the agar-gelled medium led to better growth in comparison with the control. Embryos cultured in liquid medium grew better but the germination percentage was apparently lower compared with agar-gelled medium. Liquid medium with sponge support could increase the percentage of germinated isolated embryos, but the embryo growth was not comparable to the liquid medium only. The addition of polyethylene glycol 6000 to the liquid medium seemed to increase the germination percentage and had no adverse effect on the growth of isolated embryos. Light could influence embling growth in different ways.
Starting Page	191
Ending Page	197
Page Count	7
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	38
Issue Number	2
e-ISSN	14752689
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	embryo culture germination plant conversion media optimization Cell Biology Developmental Biology Plant Genetics & Genomics Plant Biochemistry Plant Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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