NDLI: Synergism of polyamines and plant growth regulators enhanced morphogenesis, stevioside content, and production of commercially important natural antioxidants in Stevia rebaudiana Bert

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In Vitro Cellular & Developmental Biology - Plant : Volume 52, Issue 1, February 2016

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Synergism of polyamines and plant growth regulators enhanced morphogenesis, stevioside content, and production of commercially important natural antioxidants in Stevia rebaudiana Bert

Content Provider	Springer Nature Link
Author	Khalil, Shahid Akbar Kamal, Nasir Sajid, Muhammad Ahmad, Nisar Zamir, Roshan Ahmad, Naveed Ali, Shahid
Copyright Year	2016
Abstract	This study was aimed to evaluate the synergistic effects of polyamines (PAs) and plant growth regulators (PGRs) on in vitro propagation and stevioside production in Stevia rebaudiana (Stevia). A large-scale in vitro propagation protocol was established for leaf explants on Murashige and Skoog medium (MS) supplemented with various combinations of PAs and PGRs. The synergistic combination of spermidine (Spd, 2.0 mg L$^{−1}$) with 2, 4-dichlorophenoxy acetic acid (2, 4-D, 1.5 mg L$^{−1}$) and 6-benzyleadenine (BA, 1.5 mg L$^{-1}$) induced maximum callogenic response (91.7%). The combination of Spd (1.0 mg L$^{−1}$) and BA (1.0 mg L$^{−1}$) was found most effective for shoot regeneration (94.4%), mean number of shoots (14. 7), and leaves per explant (88.3). However, the combination of putrescine (Put, 2.0 mg L$^{−1}$) and kinetin (Kn, 2.0 mg L$^{−1}$) promoted mean shoot length (6.6 cm). Incorporation of either Spd or Put in combination with naphthalene acetic acid (NAA) or indole butyric acid (IBA) to culture media improved root organogenesis. Vigorous plantlets having optimum roots were successfully acclimatized in soil. Chromatographic data revealed that the synergism of Spd, BA, and Kn (2.0 mg L$^{−1}$) enhanced stevioside content in shoots (10.20 mg/g DSB) as compared to control (3.02 mg/g DW). Furthermore, application of Put and BA (2.0 mg L$^{−1}$) enhanced fresh (57.5 g L$^{−1}$ FSB) and dry shoot biomass (9.03 g L$^{−1}$ DSB) compared to control. In contrast, the Spd and BA (2.0 mg L$^{−1}$) increased antioxidant activity (80.6%) as compared to control (55.3%). Combination of Spd, BA, and GA$_{3}$ (2.0 mg L$^{-1}$) enhanced the production of phenolic and flavonoid contents. This is the first successful report on the application of polyamines for large-scale in vitro propagation and accumulation of higher stevioside content, a potential step towards industrial production.
Starting Page	174
Ending Page	184
Page Count	11
File Format	PDF
ISSN	10545476
Journal	In Vitro Cellular & Developmental Biology - Plant
Volume Number	52
Issue Number	2
e-ISSN	14752689
Language	English
Publisher	Springer US
Publisher Date	2016-02-22
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Morphogenesis Putrescine Stevia Stevioside Spermidine Phenolics Plant Sciences Cell Biology Developmental Biology Plant Breeding/Biotechnology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Plant Science Biotechnology

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