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| Content Provider | Springer Nature Link |
|---|---|
| Author | Li, Dong Wang, Man Cheng, Shaoze Zhang, Chen Wang, Yilin Zhang, Wenhui Zhao, Ruifeng Sun, Changhua Zhang, Yani Li, Bichun |
| Copyright Year | 2017 |
| Abstract | This study aimed to explore the regulatory mechanism of metabolism of xenobiotics by cytochrome P450 during the differentiation process of chicken embryonic stem cells (ESCs) into spermatogonial stem cells (SSCs) and consummate the induction differentiation system of chicken embryonic stem cells (cESCs) into SSCs in vitro. We performed RNA-Seq in highly purified male ESCs, male primordial germ cells (PGCs), and SSCs that are associated with the male germ cell differentiation. Thereinto, the metabolism of xenobiotics by cytochrome P450 was selected and analyzed with Venny among male ESC vs male PGC, male PGC vs SSC, and male ESC vs SSC groups and several candidates differentially expressed genes (DEGs) were excavated. Finally, quantitative real-time PCR (qRT-PCR) detected related DEGs under the condition of retinoic acid (RA) induction in vitro, and the expressions were compared with RNA-Seq. By knocking down CYP1A1, we detected the effect of CYP1A1-mediated metabolism of xenobiotics by cytochrome P450 on male germ cell differentiation by qRT-PCR and immunocytochemistry. Results showed that 17,742 DEGs were found during differentiation of ESCs into SSCs and enriched in 72 differently significant pathways. Thereinto, the metabolism of xenobiotics by cytochrome P450 was involved in the whole differentiation process of ESCs into SSCs and several candidate DEGs: CYP1A1, CYP3A4, CYP2D6, ALDH3B1, and ALDH1A3 were expressed with the same trend with RNA-Seq. Knockdown of CYP1A1 caused male germ cell differentiation under restrictions. Our findings showed that the metabolism of xenobiotics by cytochrome P450 was significantly different during the process of male germ cell differentiation and was persistently activated when we induced cESCs to differentiate into SSCs with RA in vitro, which illustrated that the metabolism of xenobiotics by cytochrome P450 played a crucial role in the differentiation process of ESCs into SSCs. |
| Starting Page | 293 |
| Ending Page | 303 |
| Page Count | 11 |
| File Format | |
| ISSN | 10712690 |
| Journal | In Vitro Cellular & Developmental Biology - Animal |
| Volume Number | 53 |
| Issue Number | 4 |
| e-ISSN | 1543706X |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2017-03-31 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | RNA-Seq Embryonic stem cells Primordial germ cells Spermatogonial stem cells Metabolism of xenobiotics by cytochrome P450 Differentiation Cell Biology Developmental Biology Stem Cells Cell Culture Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Medicine |
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