NDLI: Ergovaline movement across caco-2 cells

Content Provider	Springer Nature Link
Author	Shappell, Nancy W. Smith, David J.
Copyright Year	2005
Abstract	Ergovaline's role in the direct causation of fescue toxicosis first requires establishment of its dietary absorption. Therefore, ergovaline movement across human intestinal cells was assessed using Caco-2 cells derived from human colon carcinoma. A pre-equilibrated mixture of ergovaline/ergovalinine (60∶40 ratio of isomers) was added to the apical compartment, and isomer movements were assessed by high-performance liquid chromatography (HPLC) of extracted media (initial pre-isomerized ergovaline concentrations of 6 and 22 μM, two doses). Mathematical models for ergot alkaloid movement were developed. Rates of movement were not different for the isomers. In the absence of cells, basal accumulation of isomers was essentially linear for 3 h regardless of loading concentration, after which basal accumulation of ergovaline/ergovalinine plateaued. Rates of ergovaline/ergovalinine movement in the presence of cells slowed to about 25% the rate of movement in the absence of cells (22 μM, kt=0.0133 no cells, 0.0036 with cells, P<0.05). Mass transfer rate was 7.5 ng·cm$^{−2}$·min$^{−1}$ and was similar to that reported for ergovaline using a parabiotic chamber with sheep omasum. After 6 h in the presence of cells, ∼25 and 40% of the total ergovaline/ergovalinine administered had accumulated in the basal compartment for 6.6 and 22 μM treatments, respectively. Ergovaline and its naturally occurring isomer, ergovalinine, readily crossed intestinal cells intact and at similar rates. Either isomer, or a combination of both, could be involved in the pathogenesis of fescue toxicosis at sites distal to the intestine.
Starting Page	245
Ending Page	251
Page Count	7
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	41
Issue Number	8-9
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	ergopeptine fescue toxicosis intestinal cells Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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