NDLI: Human amniotic fluid-derived stem cells can differentiate into hepatocyte-like cells in vitro and in vivo

Content Provider	Springer Nature Link
Author	Liu, Hui Liu, Da qing Li, Bao wei Guan, Li dong Yan, Zhi feng Li, Ya li Pei, Xue tao Yue, Wen Wang, Min Lu, Yan ping Peng, Hong mei Lv, Yang
Copyright Year	2011
Abstract	Although human amniotic fluid is an attractive source of multipotent stem cells, the potential of amniotic fluid stem cells (AFSCs) to differentiate into hepatic cells has not been extensively evaluated. In this study, we examined whether human AFSCs can differentiate into a hepatic cell lineage in vitro and in vivo. After being treated with cytokines (fibroblast growth factor 4, basic fibroblast growth factor, hepatocyte growth factor, and oncostatin), AFSCs developed a morphology similar to that of hepatocytes. RT-PCR and immunofluorescence analysis showed that the treated AFSCs expressed the hepatocyte-specific markers albumin, cytokeratin 18, and alpha-fetoprotein. The differentiated cells also developed hepatocyte-specific functions, i.e., they secreted albumin, absorbed indocyanine green, and stored glycogen. When transplanted into CCl$_{4}$-injured immunodeficient mice, undifferentiated AFSCs were integrated into the liver tissue, and they expressed markers characteristic of mature human hepatocytes. Although integration of AFSCs into the liver was limited (0.1–0.3% of hepatocytes), histological analysis showed that the recipient mice recovered more rapidly from CCl$_{4}$ injury than CCl$_{4}$-injured mice that did not receive AFSCs. AFSCs can differentiate into hepatocyte-like cells in vitro and in vivo and can represent an easily accessible source of progenitor cells for hepatocyte regeneration and liver cell transplantation.
Starting Page	601
Ending Page	608
Page Count	8
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	47
Issue Number	9
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-09-22
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Amniotic fluid Stem cells Hepatic differentiation Cell transplantation Animal Genetics and Genomics Developmental Biology Cell Culture Cell Biology Stem Cells
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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