NDLI: Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates

Content Provider	Springer Nature Link
Author	Dike, Laura E. Chen, Christopher S. Mrksich, Milan Tien, Joe Whitesides, George M. Ingber, Donald E.
Copyright Year	1999
Abstract	Past studies using micropatterned substrates coated with adhesive islands of extracellular matrix revealed that capillary endothelial cells can be geometrically switched between growth and apoptosis. Endothelial cells cultured on single islands larger than 1500 µm$^{2}$ spread and progressed through the cell cycle, whereas cells restricted to areas less than 500 µm$^{2}$ failed to extend and underwent apoptosis. The present study addressed whether island geometries that constrained cell spreading to intermediate degrees, neither supporting cell growth nor inducing apoptosis, cause cells to differentiate. Endothelial cells cultured on substrates micropatterned with 10-µm-wide lines of fibronectin formed extensive cell-cell contacts and spread to approximately 1000 µm$^{2}$. Within 72 h, cells shut off both growth and apoptosis programs and underwent differentiation, resulting in the formation of capillary tube-like structures containing a central lumen. Accumulation of extracellular matrix tendrils containing fibronectin and laminin beneath cells and reorganization of platelet endothelial cell adhesion molecule-positive cell-cell junctions along the lengths of the tubes preceded the formation of these structures. Cells cultured on wider (30-µm) lines also formed cell-cell contacts and aligned their actin cytoskeleton, but these cells spread to larger areas (2200 µm$^{2}$), proliferated, and did not form tubes. Use of micropatterned substrates revealed that altering the geometry of cell spreading can switch endothelial cells among the three major genetic programs that govern angiogenesis—growth, apoptosis and differentiation. The system presented here provides a well-defined adhesive environment in which to further investigate the steps involved in angiogenesis.
Starting Page	441
Ending Page	448
Page Count	8
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	35
Issue Number	8
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	capillary tubes morphogenesis cell adhesion extracellular matrix microfabrication Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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© 1999 Society for In Vitro Biology 1071-2690/99 $05.00+0.00 GEOMETRIC CONTROL OF SWITCHING BETWEEN GROWTH, APOPTOSIS, AND DIFFERENTIATION DURING ANGIOGENESIS USING MICROPATTERNED SUBSTRATES (1999)

Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates

© 1999 Society for In Vitro Biology 1071-2690/99 $05.00+0.00 GEOMETRIC CONTROL OF SWITCHING BETWEEN GROWTH, APOPTOSIS, AND DIFFERENTIATION DURING ANGIOGENESIS USING MICROPATTERNED SUBSTRATES (1999)

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Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates

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© 1999 Society for In Vitro Biology 1071-2690/99 $05.00+0.00 GEOMETRIC CONTROL OF SWITCHING BETWEEN GROWTH, APOPTOSIS, AND DIFFERENTIATION DURING ANGIOGENESIS USING MICROPATTERNED SUBSTRATES (1999)

Geometric control of switching between growth, apoptosis, and differentiation during angiogenesis using micropatterned substrates

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