NDLI: Proteasome activity in a naïve mosquito cell line infected with Wolbachia pipientis wAlbB

Content Provider	Springer Nature Link
Author	Fallon, Ann M. Witthuhn, Bruce A.
Copyright Year	2009
Abstract	We used Wolbachia pipientis strain wAlbB from Aedes albopictus Aa23 cells to infect clonal Ae. albopictus TK-6 cells, which are resistant to 5-bromodeoxyuridine. Infected TK-6 cells were cultured in medium containing 5-bromodeoxyuridine to select against Aa23 cells that might have persisted in the inoculum. Infected TK-6 lines retained the Wolbachia infection for 5 mo, indicating that their metabolic processes support Wolbachia growth and multiplication. To investigate early events after Wolbachia infection, we labeled infected cells with $^{35}$S[methionine/cysteine]. Patterns of labeled proteins on sodium dodecyl sulfate gels were similar in control and infected cells, with the exception of a 29-kDa protein. Tandem mass spectrometry revealed that the 29-kDa band included α and β subunits of the 26S proteasome. Independent confirmation of the up-regulation of the proteasome was established by probing Western blots with a monoclonal antibody to the proteasome-associated co-factor, ubiquitin. Wolbachia’s loss of metabolic pathways for the synthesis of most amino acids and retention of pathways for their uptake and metabolism suggest that proteasome activation provides a mechanism whereby controlled degradation of intracellular host proteins would increase availability of amino acids to support establishment and maintenance of the Wolbachia infection.
Starting Page	460
Ending Page	466
Page Count	7
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	45
Issue Number	8
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-03-19
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Wolbachia Aedes albopictus Mosquito cell line Proteasome Ubiquitin Mass spectrometry Animal Genetics and Genomics Cell Culture Stem Cells Developmental Biology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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Cytological properties of an Aedes albopictus mosquito cell line infected with Wolbachia strain wAlbB

Proteasome activity in a naïve mosquito cell line infected with Wolbachia pipientis wAlbB.

The oxidizing agent, paraquat, is more toxic to Wolbachia than to mosquito host cells

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Proteasome activity in a naïve mosquito cell line infected with Wolbachia pipientis w AlbB

Cytological properties of an Aedes albopictus mosquito cell line infected with Wolbachia strain wAlbB

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Standardization of a colorimetric method to quantify growth and metabolic activity of Wolbachia-infected mosquito cells

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