NDLI: Persistence of TGF-β1 induction of increased fibroblast contractility ( In Vitro Cellular & Developmental Biology

Content Provider	Springer Nature Link
Author	Liu, X. D. Umi, T. Ertl, R. Veys, T. Skold, C. M. Takigawa, K. Romberger, D. J. Spurzem, J. R. Zhu, Y. K. Kohyama, T. Wang, H. Rennard, S. I.
Copyright Year	2001
Abstract	Fibroblast contraction of collagen gels is regarded as a model of wound contraction. Transforming growth factor (TGF)-β added to such gels can augment contraction consistent with its suggested role as a mediator of fibrotic repair. Since fibroblasts isolated from fibrotic tissues have been suggested to express a “fibrotic phenotype”, we hypothesized that TGF-β exposure may lead to a persistent increase in fibroblasts' contractility. To evaluate this question, confluent human fetal lung fibroblasts were treated with serum-free Dulbecco modified Eagle medium (DMEM), with or without 100 nM TGF-β1, TGF-β2, or TGF-β3 for 48 h. Fibroblasts were then trypsinized and cast into gels composed of native type I collagen isolated from rat tail tendons. After 20 min for gelation, the gels were released and maintained in serum-free DMEM. TGF-β-pretreated fibroblasts caused significantly more rapid gel contraction (52.5±0.6, 50.9±0.2, and 50.3±0.5% by TGF-β1,-β2, and-β3 pretreated fibroblasts, respectively) than control fibroblasts (74.0±0.3%, P<0.01). This effect is concentration dependent (50–200 nM), and all three isoforms had equal activity. The effect of TGF-β1, however, persisted for only a short period of time following the removal of TGF-β, and was lost with sequential passage. These observations suggest that the persistent increase in collagen-gel contractility, mediated by fibroblasts from fibrotic tissues, would not appear to be solely due to previous exposure of these cells to TGF-β.
Starting Page	193
Ending Page	201
Page Count	9
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	37
Issue Number	3
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	TGf-β fibrosis Collagen gel lung Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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