NDLI: Derivation, culture, and characterization of VUB hESC lines

Content Provider	Springer Nature Link
Author	Mateizel, Ileana Spits, Claudia Rycke, Martine Liebaers, Inge Sermon, Karen
Copyright Year	2010
Abstract	In this report, we present the derivation and characterization of 15 hESC lines established at the Vrije Universiteit Brussel, Belgium in collaboration with the Universitair Ziekenhuis Brussel, Belgium, using surplus in vitro fertilization embryos and embryos carrying monogenic disorders donated for research. Four lines were derived from blastocyst-stage embryos presumed to be genetically normal, and 11 hESC lines were obtained from embryos shown to carry genetic mutations by preimplantation genetic diagnosis. All the lines express markers of pluripotency as determined by immunocytochemistry and RT-PCR, and formed teratomas when injected into SCID mice. All VUB hESC lines, except for VUB17, are reported in the European hESC registry and are available upon request after signing a Material Transfer Agreement from the VUB (contact person: Prof. Dr. Karen Sermon; Karen.Sermon@uzbrussel.be).
Starting Page	300
Ending Page	308
Page Count	9
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	46
Issue Number	3-4
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-03-12
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	hESC Preimplantation genetic diagnosis Animal Genetics and Genomics Cell Culture Stem Cells Developmental Biology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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