NDLI: Primary and continuous midgut cell cultures fromPseudaletia unipuncta (lepidoptera: Noctuidae)

Content Provider	Springer Nature Link
Author	Garcia, Juan J. Li, Guoxun Wang, Ping Zhong, Jiang Granados, Robert R.
Copyright Year	2001
Abstract	Midgut epithelial cells were isolated from fifth-instarPseudaletia unipuncta larvae by collagenase treatment of midgut tissue, and cultured in TNM-FH medium. Long-term continuous culture and maintenance of midgut cells were achieved withP. unipuncta armyworm intestinal cells. Several cells lines were obtained from theseP. unipuncta primary cultures, and they have been subcultured and maintained for over 24 mo. The three major midgut cell types were present in the cultures, including stem (regenerative), columnar, and goblet cells. In vitro morphogenesis and differentiation of columnar and goblet cells from stem cells were observed. There appeared to be a cycle of cell death of goblet and columnar cells followed by their replacement from stem cells every 7–8 wk. After approximately six passages, the cell density in T-flasks appeared to be somewhat constant, reaching 10$^{3}$–10$^{4}$ cells per milliliter of medium. The columnar cells are round to rectangular in shape and possess a brush border, while the goblet cells have a classic flask-like shape with a central cavity. Peritrophic membrane-like secretions were observed in all the culture flasks. Infection of these cells with multiply embedded nucleopolyhedrovirus was confirmed, and we conclude that these midgut cells can be used as an in vitro model system to study early events in baculovirus infection.
Starting Page	353
Ending Page	359
Page Count	7
File Format	PDF
ISSN	10712690
Journal	In Vitro Cellular & Developmental Biology - Animal
Volume Number	37
Issue Number	6
e-ISSN	1543706X
Language	English
Publisher	Springer-Verlag
Publisher Date	2001-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Pseudaletia unipuncta midgut epithelium insect cell culture Cell Biology Developmental Biology Animal Genetics and Genomics Animal Biochemistry Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine

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