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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ma, Changping Song, Huibin Guan, Kaifeng Zhou, Jiawei Xia, Xuanyan Li, Fenge |
| Copyright Year | 2016 |
| Abstract | Swine testicular (ST) cell line is isolated from swine fetal testes and has been widely used in biomedical research fields related to pig virus infection. However, the potential benefit and utilization of ST cells in boar reproductive studies has not been fully explored. As swine fetal testes mainly contain multiple types of cells such as Leydig cells, Sertoli cells, gonocytes, and peritubular myoid cells, it is necessary to clarify the cell type of ST cell line. In this study, we identified ST cell line was a collection of Sertoli cells by analyzing the unique morphological characteristic with satellite karyosomes and determining the protein expression of two markers (androgen-binding protein, ABP; Fas ligand, FASL) of Sertoli cells. Then ST cells were further confirmed to be immature Sertoli cells by examining the expression of three markers (anti-Mullerian hormone, AMH; keratin 18, KRT18; follicle-stimulating hormone receptor, FSHR). In conclusion, ST cells are a collection of immature Sertoli cells which can be good experimental materials for the researches involved in Sertoli cell functions and maturation, or even in boar reproductions. |
| Starting Page | 427 |
| Ending Page | 433 |
| Page Count | 7 |
| File Format | |
| ISSN | 10712690 |
| Journal | In Vitro Cellular & Developmental Biology - Animal |
| Volume Number | 52 |
| Issue Number | 4 |
| e-ISSN | 1543706X |
| Language | English |
| Publisher | Springer US |
| Publisher Date | 2016-01-07 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Swine testicular cell Sertoli cell Satellite karyosomes Boar reproduction Cell Biology Developmental Biology Stem Cells Cell Culture Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Medicine |
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