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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wang, Ji ye Li, Hong ye |
| Copyright Year | 2008 |
| Abstract | Agrobacterium tumefaciens-mediated transformation (ATMT) system was assessed for conducting insertional mutagenesis in Penicillium digitatum, a major fungal pathogen infecting post-harvest citrus fruits. A transformation efficiency of up to 60 transformants per 10$^{6}$ conidia was achieved by this system. The integration of the hph gene into the fungal genome was verified by polymerase chain reaction (PCR) amplification and sequencing. These transformants tested were also shown to be mitotically stable. Southern blot analysis of 14 randomly selected transformants showed that the hph gene was randomly integrated as single copy into the fungal genome of P. digitatum. Thus, we conclude that ATMT of P. digitatum could be used as an alternatively practical genetic tool for conducting insertional mutagenesis in P. digitatum to study functional genomics. |
| Starting Page | 823 |
| Ending Page | 828 |
| Page Count | 6 |
| File Format | |
| ISSN | 16731581 |
| Journal | Journal of Zhejiang University SCIENCE B |
| Volume Number | 9 |
| Issue Number | 10 |
| e-ISSN | 18621783 |
| Language | English |
| Publisher | SP Zhejiang University Press |
| Publisher Date | 2008-10-02 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Penicillium digitatum Agrobacterium tumefaciens-mediated transformation (ATMT) Hygromycin B resistance gene Insertional mutagenesis Biomedicine general |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics |
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