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| Content Provider | Springer Nature Link |
|---|---|
| Author | Liu, Xian bao Chen, Han Chen, Hui qiang Zhu, Mei fei Hu, Xin yang Wang, Ya ping Jiang, Zhi Xu, Yin chuan Xiang, Mei xiang Wang, Jian an |
| Copyright Year | 2012 |
| Abstract | Mesenchymal stem cell (MSC) transplantation is a promising therapy for ischemic heart diseases. However, poor cell survival after transplantation greatly limits the therapeutic efficacy of MSCs. The purpose of this study was to investigate the protective effect of angiopoietin-1 (Ang1) preconditioning on MSC survival and subsequent heart function improvement after transplantation.MSCs were cultured with or without 50 ng/ml Ang1 in complete medium for 24 h prior to experiments on cell survival and transplantation. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Hoechst staining were applied to evaluate MSC survival after serum deprivation in vitro, while cell survival in vivo was detected by terminal deoxynucleotidyl transferase biotin-dUPT nick end labeling (TUNEL) assay 24 and 72 h after transplantation. Heart function and infarct size were measured four weeks later by small animal echocardiography and Masson’s trichrome staining, respectively.Ang1 preconditioning induced Akt phosphorylation and increased expression of Bcl-2 and the ratio of Bcl-2/Bax. In comparison with non-preconditioned MSCs, Ang1-preconditioned cell survival was significantly increased while the apoptotic rate decreased in vitro. However, the PI3K/Akt pathway inhibitor, LY294002, abrogated the protective effect of Ang1 preconditioning. After transplantation, the Ang1-preconditioned-MSC group showed a lower death rate, smaller infarct size, and better heart functional recovery compared to the non-preconditioned-MSC group.Ang1 preconditioning enhances MSC survival, contributing to further improvement of heart function. |
| Starting Page | 616 |
| Ending Page | 623 |
| Page Count | 8 |
| File Format | |
| ISSN | 16731581 |
| Journal | Journal of Zhejiang University SCIENCE B |
| Volume Number | 13 |
| Issue Number | 8 |
| e-ISSN | 18621783 |
| Language | English |
| Publisher | SP Zhejiang University Press |
| Publisher Date | 2012-08-08 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Mesenchymal stem cells Angiopoietin-1 Preconditioning Survival Myocardial infarction Biomedicine general |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics |
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