NDLI: Homoharringtonine induces apoptosis of endothelium and down-regulates VEGF expression of K562 cells

Content Provider	Springer Nature Link
Author	Xiu jin, Ye Mao fang, Lin
Copyright Year	2004
Abstract	Homoharringtonine (HHT) has currently been used successfully in the treatment of acute and chronic myeloid leukemias and has been shown to induce apoptosis of different types of leukemic cells in vitro. Emerging evidence suggests that angiogenesis may play an important role in hematological malignancies, such as leukemia. However, whether HHT can relieve leukemia by anti-angiogenesis is still unknown. We investigated the anti-angiogenesis potential of HHT with the human umbilical vein endothelial cell line (ECV304) and leukemic cell line (K562) in vitro. Cellular proliferation was determined by MTT assay and apoptosis was analyzed by flow cytometry. The mRNA expression of vascular endothelial growth factor (VEGF) was assessed by RT-PCR and VEGF protein production was detected by Western blot. Inhibition of cell proliferation and induction of apoptosis by HHT were discovered in ECV304 cells, and appeared in a dose- and time-dependent manner. Also, treatment with HHT caused down-regulation of VEGF mRNA expression in K562 cells in similar dose- and time-dependent manner and inhibition of VEGF protein production in K562 cells in response to the enhancing concentration of HHT. The results demonstrated that HHT could also induce apoptosis in endothelium and down-regulate VEGF expression in K562 cells. In conclusion, we believe HHT has anti-angiogenesis potential and speculate that HHT might exert its anti-leukemia effects via reduction of angiogenesis.
Starting Page	230
Ending Page	234
Page Count	5
File Format	PDF
ISSN	1673565X
Journal	Journal of Zhejiang University SCIENCE A
Volume Number	5
Issue Number	2
e-ISSN	18621775
Language	English
Publisher	Zhejiang University Press
Publisher Date	2004-02-01
Publisher Place	Hangzhou
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Homoharringtonine (HHT) Leukemia Angiogenesis VEGF Mechanical Engineering Civil Engineering Classical Continuum Physics Industrial Chemistry/Chemical Engineering
Content Type	Text
Resource Type	Article
Subject	Engineering

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