NDLI: Molecular cloning and nucleotides sequence analysis of G$_{1}$ genome segment of hantaan virus Z$

Content Provider	Springer Nature Link
Author	Wei feng, Liang Yue hong, Shen Yi lin, Ma Nian feng, Zhao Ya gang, Chen Zhi yong, Zhu Xiao, Xu
Copyright Year	2000
Abstract	The molecular cloning of the G$_{1}$ genome segment of the Z$_{10}$ strain of Hantaan virus and analysis of the first gene data on the currently widely used Hantavirus vaccine strain in China are presented. The G$_{1}$ genome segment of Z$_{10}$ virus was amplified by the method of RT-PCR, and the products were cloned into the pGEM-T vector after identification and purification. The clone was sequenced by Sanger’s dideoxy chain termination method. The 1449 bases of the Z$_{10}$ G$_{1}$ genome segment, and the coding for 483 amino acids were determined. The base compositions for the G$_{1}$ segment RNA determined from cDNA sequence information, were 20.6% A, 21.6% G, 18.8% C and 30.0% U. These values are similar to those of the 76/118, Lee and SR virus. As compared to the Z$_{10}$ G$_{1}$ segment, the sequence homology at the nucleotide level is 87% (76/118, type I), 86% (Lee, type I), 86% (Hojo, type I), 67% (R22, type II), and 59% (K22, type III). The Z$_{10}$ virus G$_{1}$ protein amino acid sequence identity with other Hantaan viruses (94–95% homology) was higher than that with Seoul type viruses (77–80%). More amino acid sequence heterogeneity between the Z$_{10}$ and 76/118 was observed in the N terminal, especially the diverging cluster of ten amino acids at position 84 to 93 of the Z$_{10}$ virus G$_{1}$ protein. Conclusion: (1) The Z$_{10}$ strain is one of the Hantaan viruses. (2) The important region of the Z$_{10}$ G$_{1}$ segment was conservative. (3) Although substantial divergence of nucleotide sequences of the G$_{1}$ genome segment was found between the Z$_{10}$ strain and other type I Hantaviruses, relatively high amino acid sequence homology was shown among them. Thus, good immune protection could be obtained with the inactivated Meriones unguiculatus kidney cell vaccine against other strains of Hantaviruses.
Starting Page	207
Ending Page	211
Page Count	5
File Format	PDF
ISSN	1673565X
Journal	Journal of Zhejiang University SCIENCE A
Volume Number	1
Issue Number	2
e-ISSN	18621775
Language	English
Publisher	Zhejiang University Press
Publisher Date	2000-04-01
Publisher Place	Hangzhou
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Hantaan virus reverse transcription-PCR sequence analysis Mechanical Engineering Civil Engineering Classical Continuum Physics Industrial Chemistry/Chemical Engineering
Content Type	Text
Resource Type	Article
Subject	Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Mechanical behavior of segment rebar of shield tunnel in construction stage

Detection of bacterial DNA by PCR and reverse hybridization in the 16s rRNA gene

Characterization and RNA2 nucleotide sequence of broad bean wilt virus 2 isolate P158

Emergence of a new satellite RNA from cucumber mosaic virus isolate P1

Optimal multi-degree reduction of Bézier curves with G $^{1}$-continuity

Study on effect of segments erection tolerance and wedge-shaped segment on segment ring in shield tunnel

The diagnostic significance of the detection of cytokeratin 19 mRNA by quantitative RT-PCR in benign and malignant pleural effusions

A quadratic programming method for optimal degree reduction of Bézier curves with G $^{1}$-continuity

Optimal approximate merging of a pair of Bézier curves with G$^{2}$-continuity

Molecular cloning and nucleotides sequence analysis of G$_{1}$ genome segment of hantaan virus Z$_{10}$ strain

Similar Documents

Mechanical behavior of segment rebar of shield tunnel in construction stage

Detection of bacterial DNA by PCR and reverse hybridization in the 16s rRNA gene

Characterization and RNA2 nucleotide sequence of broad bean wilt virus 2 isolate P158

Emergence of a new satellite RNA from cucumber mosaic virus isolate P1

Optimal multi-degree reduction of Bézier curves with G $^{1}$-continuity

Study on effect of segments erection tolerance and wedge-shaped segment on segment ring in shield tunnel

The diagnostic significance of the detection of cytokeratin 19 mRNA by quantitative RT-PCR in benign and malignant pleural effusions

A quadratic programming method for optimal degree reduction of Bézier curves with G $^{1}$-continuity

Optimal approximate merging of a pair of Bézier curves with G$^{2}$-continuity

Molecular cloning and nucleotides sequence analysis of G$_{1}$ genome segment of hantaan virus Z$_{10}$ strain