NDLI: New isolation method for endophytes based on enzyme digestion

Content Provider	Springer Nature Link
Author	Prior, René Görges, Katharina Yurkov, Andrey Begerow, Dominik
Copyright Year	2014
Abstract	Investigation of the diversity and ecology of endophytic fungi has gained popularity in recent decades. Thereby, culture-independent methods were very instrumental as they revealed greater species richness and challenged the efficiency of isolation methods. However, culture-based methods remain the only possibility to provide strains for future studies. To improve the efficiency of endophyte isolation, we compared seven different artificial media that included a variety of plant extracts. Furthermore, we developed a method based on enzymatic digestion of plant tissue to improve the isolation efficiency in terms of species richness. The effect of additional plant extract in media that contained yeast and malt extract was of minor significance, whereas isolations using enzymatic digestion of plant leaf tissue combined with a 1:10 dilution of nutrients revealed a much higher diversity of species. Using this protocol, we isolated three times as many species from the same leaves than with previously described methods. This is reflected by the large number of singletons obtained in culture-independent approaches to study the diversity of endophytic fungi.
Starting Page	849
Ending Page	856
Page Count	8
File Format	PDF
ISSN	1617416X
Journal	Mycological Progress
Volume Number	13
Issue Number	3
e-ISSN	18618952
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-03-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Endophytes Fungi Strain cultivation Isolation method Enzyme digestion Plant Sciences Ecology Microbiology
Content Type	Text
Resource Type	Article
Subject	Plant Science Ecology, Evolution, Behavior and Systematics

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