NDLI: Analysis of bacterial communities in and on concrete

Content Provider	Springer Nature Link
Author	Maresca, Julia A. Moser, Paul Schumacher, Thomas
Copyright Year	2016
Abstract	Bacteria are known to catalyze degradation of concrete, and have more recently been used to repair micro-cracks in or form protective biofilms on cement mortar. However, the microbial communities in and on concrete under ordinary weathering conditions have not been characterized, in part because of difficulty in extracting DNA from inside concrete specimens. Here, we report a method for extraction of nucleic acids directly from hardened concrete. Using this method and classical cultivation methods, we demonstrate that most bacteria in or on concrete belong to two taxonomic groups, that the bacterial diversity is similar on the concrete surface and in the interior, and that many bacteria in and on concrete are related to microbes found in other dry, saline, or alkaline environments. This method lays the foundation for the creation of bioindicators for concrete and may open new avenues for the fields of non-destructive evaluation and assessment of concrete structures.
Starting Page	1
Ending Page	13
Page Count	13
File Format	PDF
ISSN	13595997
Journal	Materials and Structures
Volume Number	50
Issue Number	1
e-ISSN	18716873
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-08-10
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacteria Concrete Bioindicator Non-destructive evaluation Structural Mechanics Materials Science Theoretical and Applied Mechanics Operating Procedures, Materials Treatment Civil Engineering Building Materials
Content Type	Text
Resource Type	Article
Subject	Building and Construction Mechanics of Materials Materials Science Civil and Structural Engineering

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