NDLI: Effect of flow direction on the morphological responses of cultured bovine aortic endothelial cells

Content Provider	Springer Nature Link
Author	Kataoka, N. Ujita, S. Sato, M.
Copyright Year	1998
Abstract	The effect of flow direction on the morphology of cultured bovine aortic endothelial cells is studied. Fully confluent endothelial cells cultured on glass were subjected to a fluid-imposed shear stress of 2 Pa for 20 min and 24 h using a parallel plate flow chamber. Experiments on shear flow exposure were performed for (i) one-way flow, (ii) reciprocating flow with a 30 min interval and (iii) alternating orthogonal flows with a 30 min interval. After flow exposure, the endothelial cells were fixed and F-actin filaments were stained with rhodamine phalloidin. Endothelial cells were observed and photographed by means of a microscope equipped with epifluorescence optics. The shape index (SI) and angle of cell orientation were measured, and F-actin distributions in the cells were statistically studied. Endothelial cells under the one-way flow condition showed marked elongation (SI=0.39±0.16, mean±S.D.) and aligned with the flow direction. In the case of the reciprocating (SI=0.63±0.14) and the alternating orthogonal flows (0.64±0.14), cells did not elongate so strongly as in the case of one-way flow. Although most cells in the reciprocating flow aligned with the flow direction, the cell axes in the alternate orthogonal flow distributed around a mean value of −45.1° with a large S.D. value. Endothelial cells can be expected to recognise the flow direction, and change their shape and F-actin structure.
Starting Page	122
Ending Page	128
Page Count	7
File Format	PDF
ISSN	01400118
Journal	Medical and Biological Engineering and Computing
Volume Number	36
Issue Number	1
e-ISSN	17410444
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	1998-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Endothelial cells Shear stress Flow direction Cell morphology F-actin filament Human Physiology Neurosciences Imaging Radiology Computer Applications Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering Computer Science Applications

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