NDLI: Human platelet supernatant promotes proliferation but not differentiation of articular chondrocytes

Content Provider	Springer Nature Link
Author	Kaps, C. Loch, A. Haisch, A. Smolian, H. Burmester, G. R. Häupl, T. Sittinger, M.
Copyright Year	2002
Abstract	The objective of the study was to evaluate the growth-promoting activity of human platelet supernanant on primary chondrocytes in comparison with fetal calf serum (FCS) supplemented cell culture medium. Furthermore, the differentiation potential of platelet supernatant was determined in three-dimensional artificial cartilage tissues of bovine articular chondrocytes. Proliferation of articular and nasal septal chondrocytes was assayed by incorporation of BrdU upon stimulation with ten different batches of human platelet supernatant. On bovine articular chondrocytes, all these batches were at least as growth-promoting as FCS. On nasal septal chondrocytes, nine out of ten batches revealed increased or equivalent mitogenic stimulation compared with medium supplemented with FCS. Three-dimensional culture and subsequent histological analysis of matrix formation were used to determine the differentiation properties of platelet supernatant on articular chondrocytes. Human platelet supernatant failed to induce the deposition of typical cartilage matrix components, whereas differentiation and matrix formation were apparent upon cultivation of articular chondrocytes with FCS. Proliferation assays demonstrated that human platelet supernatant stimulates growth of articular and nasal septal chondrocytes; however, platelet supernatant failed to stimulate articular chondrocytes to redifferentiate in three-dimensional chondrocyte cultures. Therefore platelet lysate may be suitable for chondrocyte expansion, but not for maturation of tissue-engineered cartilage.
Starting Page	485
Ending Page	490
Page Count	6
File Format	PDF
ISSN	01400118
Journal	Medical and Biological Engineering and Computing
Volume Number	40
Issue Number	4
e-ISSN	17410444
Language	English
Publisher	Springer-Verlag
Publisher Date	2002-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Tissue engineering Platelet supernatant Chondrocyte Differentiation Proliferation Human Physiology Neurosciences Imaging Radiology Computer Applications Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering Computer Science Applications

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