NDLI: Cloning and biological activity of an anti-tumor peptide of Tumstatin

Content Provider	Springer Nature Link
Author	Wang, Shujing Liu, Yan Lin, Xuesong Fu, Xue Xu, Jianyong Liu, Xinghan
Copyright Year	2007
Abstract	To obtain an anti-tumor peptide of Tumstatin and detect its biological activity, the nucleotide sequence encoding 185–203 amino acids (19peptide) of Tumstatin was synthesized and inserted into the fusion protein vector pTYB2. After identification by sequencing and restriction endonucleases, the recombined vector was transformed into BL-21 (DE3) E. coli competent cells. Transformed E. coli BL-21 (DE3) were induced by isopropyl-β-thiogalactopyranoside (IPTG), and then expressed. By 1,4-dithiothreitol (DTT) reduction, the soluble 19peptide was obtained from a chitin affinity chromatograph. The biological activity of 19peptide was determined by 3-[4,5-dimethylthiazol-2-y1]-2,5-diphenytetrazolium bromide (MTT) assay, cell growth curve, the effect of the ascitic fluid transfevent H22 hepatoma on mice and via histopathological slices. The purified 19peptide directly inhibited proliferation and migration of murine B16 melanoma cells, SMMC-7721hepatoma carcinoma cells and human umbilical vein endothelial cells (HUVEC). The tumor inhibition rate of mice ascitic fluid transfevent H22 hepatoma was 48.46%. Histopathological slices showed that it could promote tumor tissue necrosis and decrease the density of blood vessels. With higher anti-tumor activity, 19peptide has the potential to become a novel, potent anti-tumor agent.
Starting Page	276
Ending Page	283
Page Count	8
File Format	PDF
ISSN	16733509
Journal	Frontiers in Biology
Volume Number	2
Issue Number	3
e-ISSN	16733622
Language	English
Publisher	Higher Education Press
Publisher Date	2007-01-01
Publisher Institution	Chinese Universities
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	Tumstatin protein expression protein purification tumor therapy Life Sciences
Content Type	Text
Resource Type	Article
Subject	Ecology Genetics Ecology, Evolution, Behavior and Systematics Biotechnology

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