NDLI: Proteomics analysis of cerebral cortex in Wistar rats

Content Provider	Springer Nature Link
Author	Zhao, Xiaofeng Wen, Jingrong Wang, Shu Shi, Xuemin
Copyright Year	2008
Abstract	To analyze the protein expression pattern of the cerebral cortex in Wistar rats using the proteomics approach, proteins were separated by two-dimensional gel electrophoresis, stained with Coomassie brilliant blue and digested with trypsin. Then, we analyzed the peptide section using a matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) and identified the protein by indexing special database (SwissProt) according to the finger printing of the peptide quality. Eighty-four protein spots were identified, including metabolic enzymes, skeleton proteins, heat shock proteins, antioxidant proteins, signaling proteins, proteasome related proteins, neuron and glial specific proteins and serum associated proteins. The result of this study enriches the database of the proteome in the cerebral cortex of rats and lays a foundation for further research of neurological disorders in rat models.
Starting Page	419
Ending Page	427
Page Count	9
File Format	PDF
ISSN	16733509
Journal	Frontiers in Biology
Volume Number	3
Issue Number	4
e-ISSN	16733622
Language	English
Publisher	SP Higher Education Press
Publisher Date	2008-05-22
Publisher Institution	Chinese Universities
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	brain proteomics two-dimensional gel electrophoresis (2-DE) matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS) Life Sciences
Content Type	Text
Resource Type	Article
Subject	Ecology Genetics Ecology, Evolution, Behavior and Systematics Biotechnology

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