NDLI: Label-Free Detection of Digoxin Using Localized Surface Plasmon Resonance-Based Nanobiosensor

Content Provider	Springer Nature Link
Author	Nikfarjam, Alireza Rezayan, Ali Hossein Mohammadkhani, Ghasem Mohammadnejad, Javad
Copyright Year	2016
Abstract	The scientific community finds gold nanoparticles particularly interesting due to their great applications, especially in SPR-based analysis. So far, no contributions have been made on detecting digoxin in which GNPs and LSPR technique have been used. In the present investigation, the localized surface plasmon resonance (LSPR) property of gold nanoparticles (GNPs) was utilized to develop a novel optical biosensor for the detection of digoxin. GNPs were synthesized using the sodium citrate reduction method and then made functional using the 11-mercaptoundecanoic acid (11-MUA) ligand. The carboxyl-functionalized GNPs reacted with a monoclonal antibody of digoxin through the EDC/NHS method. To characterize each processing step in the nanobiosensor preparation, UV-Vis spectrophotometry, X-ray diffraction, field emission scanning electron microscopy (FESEM), dynamic light scattering (DLS), Fourier transform infrared (FTIR), and zeta potential tests were performed. The nanobiosensor’s performance was evaluated in the presence of different concentrations of digoxin in PBS and FBS. The synthesized GNPs size was estimated as 11.2 nm and present a LSPR peak at 520 nm. The size of 11-MUA-functionalized GNPs was 13.2 nm. The FTIR results revealed that 11-MUA ligand are linked to GNPs through thiol groups (Au-S-). The surface charge of 11-MUA and the monoclonal antibody-conjugated gold nanoparticles were −29.45 and −11.69, respectively. The limit of detection (LOD) of this LSPR-based nanobiosensor was 2 ng/ml. We have developed the new LSPR-based optical biosensor for direct and inexpensive detection of digoxin. This optical biosensor is easy to fabricate and the operation procedure has become more convenient.
Starting Page	157
Ending Page	164
Page Count	8
File Format	PDF
ISSN	15571955
Journal	Plasmonics
Volume Number	12
Issue Number	1
e-ISSN	15571963
Language	English
Publisher	Springer US
Publisher Date	2016-05-19
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nanobiosensor Localized surface plasmon resonance (LSPR) Gold nanoparticles (GNPs) Digoxin Biotechnology Nanotechnology Biophysics and Biological Physics Biochemistry
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Biochemistry Biophysics Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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