NDLI: Investigation of the thermal stability of nucleic acids and their structural components

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Investigation of the thermal stability of nucleic acids and their structural components

Content Provider	Springer Nature Link
Author	Gasan, A. I. Maleev, V. Ya. Filipsky, A. V.
Copyright Year	2011
Abstract	The thermal stability of homopolynucleotides (poly(A), poly(G), poly(C), poly(U)) and natural DNA, as well as their structural components: nucleoside (uridine), nucleotides (uridine-5′-monaphosphate, uridine-5′-diphosphate, and uridine-5′-triphosphate) and sugar (D-ribose) have been studied by the method of differential scanning microcalorimetry. The dependences of the heat flow on temperature have been obtained for the compounds having individual features in the temperature range from 20 to 400°C. All samples showed exothermic peaks at temperatures higher than 200°C (for DNA, this peak was found at a temperature of ∼160°C), which are related to processes of irreversible thermal destruction. The temperatures of thermal destruction and the effective energy of activation of this process for all compounds studied have been determined. The values of the effective heat of exothermal processes have been calculated for the polynucleotides. The experimental results indicate that there is a significant difference in the thermal stability between these homopolynucleotides and DNA, poly(G) being the most stable and DNA, the least stable. Based on the analysis of D-ribose, nucleoside, and nucleotides, it was concluded that the sugar ring is the most probable region of the destruction.
Starting Page	393
Ending Page	398
Page Count	6
File Format	PDF
ISSN	00063509
Journal	Biophysics
Volume Number	56
Issue Number	3
e-ISSN	15556654
Language	English
Publisher	SP MAIK Nauka/Interperiodica
Publisher Date	2011-08-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	DNA polynucleotides thermal destruction microcalorimetry Biophysics and Biological Physics
Content Type	Text
Resource Type	Article
Subject	Biophysics

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