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| Content Provider | Springer Nature Link |
|---|---|
| Author | Guliy, O. I. Zaitsev, B. D. Kuznetsova, I. E. Shikhabudiv, A. M. Karavaeva, O. A. Dykman, L. A. Staroverov, S. A. Ignatov, O. V. |
| Copyright Year | 2012 |
| Abstract | Phage mini-antibodies to bacterial cells of strain Azospirillum brasilense Sp245 were obtained, and the possibility of using them for detection of microbial cells with a lateral field excited piezoelectric resonator was studied. It has been found that the frequency dependences of the real and imaginary parts of electrical impedance of such a resonator loaded with a suspension of A. brasilense Sp245 cells with the mini-antibodies differ significantly from the dependences of the resonator with a control cell suspension without mini-anti-bodies. The limit of possible determination of the concentration of microbial cells is found to be 10$^{3}$ cells/mL upon interaction with mini-antibodies. It has been ascertained that detection of A. brasilense Sp245 cells with the aid of mini-antibodies is possible even in the presence of other cultures, for example, E. coli BL-Ril and A. brasilense Sp7. Therefore, it has been shown for the first time that detection of microbial cells with an electroacoustic sensor is feasible. |
| Starting Page | 336 |
| Ending Page | 342 |
| Page Count | 7 |
| File Format | |
| ISSN | 00063509 |
| Journal | Biophysics |
| Volume Number | 57 |
| Issue Number | 3 |
| e-ISSN | 15556654 |
| Language | English |
| Publisher | SP MAIK Nauka/Interperiodica |
| Publisher Date | 2012-08-25 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Azospirillum brasilense Sp245 phage mini-antibody lateral field excited piezoelectric resonator detection Biophysics and Biological Physics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biophysics |
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