NDLI: DNA-protein interaction at erythroid important regulatory elements of MEL cells byin vivo footprinting

Content Provider	Springer Nature Link
Author	Ji, Xinjun Liu, Depei Xu, Dongdong Li, Lei Wang, Jing Liang, Zhiquan
Copyright Year	2000
Abstract	Using ligation-mediated PCR method to study the status of DNA-protein interaction at hypersensitive site 2 of locus control Region and β$^{maj}$ promoter of MEL cell line before and after induction, MEL cell has been cultured and induced to differentiation by Hemin and DMSO, then the live cells have been treated with dimethyl sulfate. Ligation mediated PCR has been carried out following the chemical cleavage. The results demonstrate that before and after induction, the status of DNA-protein interaction at both hypersensitive site 2 and β$^{maj}$ promoter change significantly, indicating that distal regulatory elements (locus control region, hypersensitive sites) as well as proximal regulatory elements (promoter, enhancer) of β-globin gene cluster participate in the regulation of developmental specificity.
Starting Page	1101
Ending Page	1108
Page Count	8
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	45
Issue Number	12
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2000-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	β-globin gene HS LM-PCR in vivo footprinting MEL cell line Life Sciences Physics Chemistry/Food Science Science Geosciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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DNA-protein interaction at erythroid important regulatory elements of MEL cells byin vivo footprinting