NDLI: Antigenic epitope peptides of influenza H3N2 virus neuraminidase gene based on experiments

Content Provider	Springer Nature Link
Author	Zhong, Jing Huang, Ping Wen, MiaoHeng Liang, LiJun Zhang, Xin Tan, SongNuan Zhu, XiaoLan
Copyright Year	2012
Abstract	The neuraminidase (NA) in viral surface is one of the main subtype-specific antigen of influenza type A viruses. Neuraminidase is an enzyme to break the bonds between hemagglutinin (HA) and sialic acid to release newly formed viruses from infected cells. In this study, the H3N2 subtype virus NA genes were sequenced and NA proteins were screened for B-cell epitopes and assessed based on immunoinformatics. Based on this results, four peptides DR6, EY7, VG8 and RE8 (covering amino acid residues 151–156, 368–374, 398–405 and 428–435, respectively) of the NA protein were synthesized artificially. These peptides were used to immunize New Zealand rabbits subcutaneously to raise antisera. Experimental results showed that these four peptides were capable of eliciting antibodies against H3N2 viruses in a specific and sensitive feature, detected in vitro by enzyme-linked immunosorbent assay. Moreover, hemadsorption anti-releasing effects took place in three three-antisera-mixtures at a dilution of 1:40. Alignment using NA gene database showed that amino acid residues in these four epitope peptides were substituted at specific sites in all the NAs sequenced in this study. It was suggested that these NA epitope peptides might be used in combination with HA proteins as vaccine antigens.
Starting Page	2908
Ending Page	2913
Page Count	6
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	57
Issue Number	22
e-ISSN	18619541
Language	English
Publisher	SP Science China Press
Publisher Date	2012-08-09
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	B-cell epitope H3N2 immunoinformatics neuraminidase (NA) Earth Sciences Engineering Science Chemistry/Food Science Life Sciences Physics
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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