NDLI: Inducible expression pattern of rice Bowman-Birk inhibitor geneOsWIP1-2 and its protease inhibitory activity

Content Provider	Springer Nature Link
Author	Chen, Jun Liu, Jing Guo, Lei Qu, Lijia Chen, Zhangliang Gu, Hongya
Copyright Year	2004
Abstract	TheWIP1-2 gene was cloned from rice. It belongs to the Bowman-Birk inhibitor gene family. Northern blot showed that expression of this gene was induced by wounding and jasmonic acid (JA). It indicates that theOsWIP1 gene plays an important role in the rice defense system. TheOsWIP1-2 was cloned into pET28a and expressed inE. coli. Its expressed product was purified in the form of fusion protein and tested for the inhibitory activities against trypsin and chymotrypsin. It was found that the fusion protein could inhibit chymotrypsin, but not trypsin. It was also found that the His tag at its C-terminal affected its inhibitory activity significantly. The fusion protein with a natural C-terminal had the inhibitory activity, while no inhibitory activity was detected in the fusion protein with a (His)$_{6}$-tag at its C-terminal. This implies that extra amino acid residues at the C-terminal of OsWIP1-2 may interfere with its correct folding. The inhibitory assay indicated that the members of rice Bowman-Birk inhibitor gene family probably differentiated both in their structure and function.
Starting Page	895
Ending Page	899
Page Count	5
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	49
Issue Number	9
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2004-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	Bowman-Birk inhibitor WIP1 rice wound inducible jasmonic acids Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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