NDLI: Construction and expression of inverted configuration of retroviral vector containing intron 1 of hFIX

Content Provider	Springer Nature Link
Author	Hongwei, Wang Yun, Bao Yongna, Xin Xiaoqin, Yang Qian, Shi Daru, Lu Xinfang, Qiu Jinglun, Xue
Copyright Year	1998
Abstract	Intron was found to play an important role in improving gene expression. To improve the human factor IX(hFIX) expression level In hemophilia B gene therapy study, the retroviral vector containing intron 1 of hFIX gene was constructed in forwarded configuration, but the intron 1 was found spliced in virus particles by RT-PCR detection. So the inverted configuration vector G1NaPAi’IX was suggested and constructed on the basis of SNMBAIXm and transfected into PA317. Then C2C12 cells were transfected using the above virus supernatant and the G418-resistant clones were selected. PCR and RT-PCR detection found that intron I structure exlsted in C2C12 clones and retroviral particles. And the expression level of inverted vector was 3 times higher than that of forwarded vector. These results showed that the inverted configuration vector was in deed able to avoid splicing of intron 1 during the process of retroviral packaging and improved the expression level of hFIX protein.
Starting Page	315
Ending Page	318
Page Count	4
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	43
Issue Number	4
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	1998-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	hFIX cDNA intron 1 retroviral vector gene therapy Life Sciences Physics Chemistry/Food Science Science Geosciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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