NDLI: Construction and identification of reverse genetics system of Dengue type 2 virus isolated in China

Content Provider	Springer Nature Link
Author	Zhu, Wuyang Chen, Shuiping Qin, Chenggeng Yu, Man Jiang, Tao Deng, Yongqiang Qin, Ede
Copyright Year	2006
Abstract	To construct infectious full-length cDNA clone of dengue virus type 2 isolated in China (DEN2-43), according to the published nucleotide sequence of the virus strain, the approximately 11 kb full-length cDNAs of DEN2-43 were amplified by long RT-PCR and fusion PCR. Full-length cDNA clones were constructed by inserting the full-length cDNA into a low copy vector pWSK29, from which rescued virus D212 was acquired by transcription in vitro and electroporation. The full-length cDNA clone pD212 was infectious, and rescued virus acquired in C6/36 cells was indistinguishable from DEN2-43 virus in biological properties including suckling mice neurovirulence. The reverse genetics system helps elucidate the mechanism of pathogenesis of dengue virus and develop novel vaccine against dengue.
Starting Page	2065
Ending Page	2071
Page Count	7
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	51
Issue Number	17
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2006-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	dengue virus reverse genetics system rescued virus Science Chemistry/Food Science Geosciences Life Sciences Physics Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Construction and identification of reverse genetics system of Dengue type 2 virus isolated in China