NDLI: Distribution of highly repeated DNA sequences in Haynaldia villosa and its application in the identification of alien chromatin

Content Provider	Springer Nature Link
Author	Zhang, Wei Zhang, RuiQi Feng, YiGao Bie, TongDe Chen, PeiDu
Copyright Year	2013
Abstract	Haynaldia villosa (L.) is a wild relative species of common wheat that possesses many beneficial genes that can be used for wheat improvement. The accurate detection of H. villosa chromosomes in the genetic background of wheat is critical for transferring its beneficial genes to common wheat by chromosome engineering. The aim of the present study was to investigate the distribution patterns of two repeated DNA sequences, pSc119.2 and pAs1, as well as two rDNA multigene family sequences, 45S rDNA and 5S rDNA, in the individual chromosomes of H. villosa for the future precise identification of alien chromatin in germplasm development and breeding programs. A set of common wheat-H. villosa disomic addition 1V-7V lines was used to determine these specific signals on individual chromosomes of H. villosa. The results showed that two rDNA probes, pTa71 (45S rDNA) and pTa794 (5S rDNA), were located on 1VS and 5VS, respectively, and the signal could be discriminated exclusively in the common wheat background as effective markers of 1VS and 5VS. Furthermore, all seven chromosomes of H. villosa could be distinguished clearly by fluorescence in situ hybridization using pSc119.2 and pAs1 as probes in combination. The utilization of these cytogenetic markers of repetitive sequences, combined with other molecular markers sometimes, will make it possible for a precise identification of alien chromosomes with high efficiency.
Starting Page	890
Ending Page	897
Page Count	8
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	58
Issue Number	8
e-ISSN	18619541
Language	English
Publisher	SP Science China Press
Publisher Date	2013-01-11
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	fluorescence in situ hybridization Haynaldia villosa repeated DNA sequence wheat Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Distribution of highly repeated DNA sequences in Haynaldia villosa and its application in the identification of alien chromatin

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