NDLI: Dynamic organization of actin cytoskeleton during the polarity formation and germination of pollen protoplasts

Content Provider	Springer Nature Link
Author	Xu, Xia Zi, Huijun Sun, Yina Ren, Haiyun
Copyright Year	2004
Abstract	The formation of the polarity of pollen protoplast and the dynamics of actin cytoskeleton were observed by non-fixation, Alexa-Phalloidin probing and confocal laser scanning microscopy. Our results showed that the protoplast obtained from stored pollen contained numerous crystalline fusiform bodies to constitute a storage form of actin. When dormant pollen was hydrated, the actin cytoskeleton forms a fine network spreading uniformly in the protoplast. In the process of polarity formation and germination of pollen protoplast, actin filaments marshaled slowly to the brim, and then formed multilayer continuous actin filament bundles surrounding the cortical of the protoplast. When the protoplast was exposed to actin filament-disrupting drugs, such as Latrunculin A and Cytochalasin D, continuously arranged actin bundles were disturbed and in this condition, the protoplast could not germinate. But when exposed to actin filament stabiling drug-phalliodin, the dynamics of actin filaments in the protoplasts behaved normally and the protoplasts could germinate normally. These results were also confirmed by the pharmacology experiments on pollen grains. And when Latrunculin A or Cytochalasin D was washed off, the ratio of pollen germination was resumed partly. All the results above show that the dynamic organization of the actin cytoskeleton are critical in the cell polarity formation and germination of pollen protoplast, and that the reorganization of actin cytoskeleton is mainly due to the rearrangement of actin filament arrays.
Starting Page	1702
Ending Page	1706
Page Count	5
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	49
Issue Number	16
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2004-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	actin cytoskeleton pollen protoplast Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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