NDLI: Interactions between HMG proteins (HMG1/2 and HMG14/17) and human ε-globin gene promoter (ε-promoter)

Content Provider	Springer Nature Link
Author	Hou, Chunhui Huang, Jian Zhang, Shubing Qian, Ruolan
Copyright Year	2002
Abstract	High mobility group (HMG) proteins are abundant non-histone proteins in the nuclei of eukaryocytes. It has been shown that HMG proteins may play important roles in the structure and function of chromatin. In the present study, the binding of HMG proteins (HMG1/2 and HMG14/17) to the human ε-globin gene promoter (ε-promoter, −177–+1 bp) has been examined by using both the in vitro nucleosome reconstitution and the electrophoresis mobility shift assay (EMSA). We found that HMG1/2 proteins could bind to the naked ε-promoter DNA, however, HMG14/17 could not. Using the in vitro nucleosome reconstitution, we revealed that HMG14/17 could bind to the mononucleosome reconstituted in vitro with ε-promoter, while HMG1/2 could not. Those results indicate that the binding of HMG proteins to ε-promoter is dynamic as the nucleosome assembling and disassembling. We speculated that this selective binding of HMG proteins to ε-promoter might play a critical role in the regulation of ε-globin gene expression.
Starting Page	1543
Ending Page	1546
Page Count	4
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	47
Issue Number	18
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2002-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	HMG proteins human ε-globin gene promoter in vitro nucleosome reconstitution HMG proteins purification EMSA Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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The In Vitro Reconstitution of Nucleosome and its Binding Patterns with HMG1/2 and HMG14/17 Proteins

Interactions between the nuclear matrix proteins and the 5′-flankingcis-acting elements of the human ε-globin gene

Effects of point mutation C→T at −64 of human δ globin gene promoter on DNA binding proteins

A novel erythroid differentiation related gene EDRF2 inhibited α-globin gene expression in K562 cells

Acetylation of a specific promoter nucleosome accompanies activation of the ε-globin gene by -globin locus control region hs2 (2000).

Analysis of nucleosome positioning in promoters of miRNA genes and protein-coding genes

Interactions between GATA transcription factors and the DNaseI hypersensitive sites in the human β-globin gene LCR

Roles of histones and nucleosomes in gene transcription

Relationships of mRNA-protein secondary structures in the human β-globin gene HBB and four variants

Interactions between HMG proteins (HMG1/2 and HMG14/17) and human ε-globin gene promoter (ε-promoter)

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The In Vitro Reconstitution of Nucleosome and its Binding Patterns with HMG1/2 and HMG14/17 Proteins

Interactions between the nuclear matrix proteins and the 5′-flankingcis-acting elements of the human ε-globin gene

Effects of point mutation C→T at −64 of human δ globin gene promoter on DNA binding proteins

A novel erythroid differentiation related gene EDRF2 inhibited α-globin gene expression in K562 cells

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Interactions between HMG proteins (HMG1/2 and HMG14/17) and human ε-globin gene promoter (ε-promoter)