NDLI: Cloning and characterization of a gene encoding phagerelated tail protein (PrTP) of endosymbiont Wolbachia

Content Provider	Springer Nature Link
Author	Kang, Lin Sun, Lei Yu, Hong Zhou, Weiguo Zhao, Shouyuan Li, Changben
Copyright Year	2002
Abstract	Wolbachia is an obligatory, maternally inherited intracellular bacterium, known to infect a wide range of arthropods. It has been implicated in causing cytoplasmic incompatibility (CI), parthenogenesis, the feminization of genetic males and male-killing in different hosts. However, the molecular mechanisms by which this fastidious bacterium causes these reproductive abnormalities have not yet been determined. In this study, we report on the cloning and characterization of the gene encoding phage-related tail protein (PrTP) from Wolbachia in Drosophila melanogaster CantonS (wMelCS) and from Wolbachia in Drosophila melanogaster yw67c23 (wMel) by representational difference analysis (RDA) and ligation-mediated PCR (LM-PCR). The functionality of a bipartite nuclear localization signal sequence (NLS) of the gene was also successfully tested in Drosophila S2 cells. PrTP expression in various strains of Wolbachia was investigated. Our results suggest that PrTP may not induce CI directly. However, the existence of prtp provided direct evidence of phage-mediated horizontal gene transfer (HGT) that might play an important role in a variety of reproductive abnormalities of Wolbachia.
Starting Page	1451
Ending Page	1456
Page Count	6
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	47
Issue Number	17
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2002-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	Wolbachia CI prtp NLS HGT Science Life Sciences Physics Chemistry/Food Science Geosciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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