Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Springer Nature Link |
|---|---|
| Author | Li, TianXing YiZhou Zhang, Yue Liu, Hua Wu, YuTing Li, WenBin Zhang, HongXia |
| Copyright Year | 2010 |
| Abstract | The Arabidopsis vacuolar Na$^{+}$/H$^{+}$ antiporter gene, AtNHX1, was introduced into soybean by Agrobacterium-mediated transformation. Four independent kanamycin resistant lines were obtained. The result of PCR, Southern blotting and Northern blotting analyses demonstrated that the AtNHX1 gene was successfully inserted into the soybean genome and stably expressed in these kanamycin resistant lines. The stability of AtNHX1 expression and salt resistance were evaluated in the soybean transformants for over 6 generations. Two independently derived transgenic lines with high expression level of AtNHX1 were selected, and propagated to generation T$_{5}$ in the absence of selection pressure. PCR and RT-PCR examinations revealed that AtNHX1 was highly expressed in all investigated transgenic T$_{5}$ progenies. Furthermore, all transgenic T$_{5}$ plants showed resistant to salt stress, same as those of homozygous T$_{2}$ plants. Taken together, our results indicated that constitutive expression of AtNHX1 enhanced salt tolerance in soybean for over 6 generations, suggesting a great potential use of AtNHX1 for improving salt tolerance in plants by genetic engineering. |
| Starting Page | 1127 |
| Ending Page | 1134 |
| Page Count | 8 |
| File Format | |
| ISSN | 10016538 |
| Journal | Chinese Science Bulletin |
| Volume Number | 55 |
| Issue Number | 12 |
| e-ISSN | 18619541 |
| Language | English |
| Publisher | SP Science China Press |
| Publisher Date | 2010-04-21 |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | AtNHX1 fatty acids salt tolerance soybean transgenic plants Engineering Earth Sciences Chemistry/Food Science Physics Life Sciences Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|