NDLI: Transgene directionally integrated into C-genome of Brassica napus

Content Provider	Springer Nature Link
Author	Li, Jun Fang, Xiaoping Wang, Zhuan Li, Jun Luo, Lixia Hu, Qiong
Copyright Year	2006
Abstract	Integration of a transgene into a C-genome chromosome plays an important role in reducing ecological risk of transgenic Brassica napus. To obtain C-genome transgenic B. napus, herbicide-resistant bar gene was firstly transferred into B. oleracea var. alboglabra mediated by Agrobacterium tumefaciens strain LBA4404. Then using the transgenic B. oleracea as paternal plants and 8 non-transgenic varieties of B. rapa as maternal plants, C-genome transgenic B. napus with bar gene was artificially resynthesized by means of ovary culture and chromosome doubling. Among 67 lines of the resynthesized B. napus, 31 were positive, and 36 were negative according to PCR test for bar gene. At least 2 plants from each line were kept for PPT spray confirmation. The result was in consistence with the PCR test. Genomic Southern blotting of three randomly chosen lines also showed that bar gene had been integrated into the genome of resynthesized B. napus lines.
Starting Page	1578
Ending Page	1585
Page Count	8
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	51
Issue Number	13
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2006-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	Bar gene C-genome directional transgene resynthesis of Brassica napus Chemistry/Food Science Geosciences Life Sciences Physics Engineering Science
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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