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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wu, Danli Zhang, Yourong Lao, Miaofen Qiu, Zhaohua Ha, Xiaoqin Wu, Zuze Wu, Cutse |
| Copyright Year | 2003 |
| Abstract | In order to investigate the feasibility of myocardial ischemia gene therapy, we cloned human hepatocyte growth factor gene from human placenta cDNA library by the RT-PCR method. Recombination adenovirus Ad-HGF was constructed by the method of co-transfection and homologous recombination of plasmids in 293 cells. Ad-HGF was amplified in 293 cells and purified through CsCl density gradient centrifugation. Ad-HGF could be expressed in rat primary myocardial cells and HGF secreted into the culture media, which was tested by ELISA. The distribution and persistence of adenovirus in rat were investigated by green fluorescence protein as a report gene.In vivo we found that intramyocardial administration of Ad-HGF could induce angiogenesis in rat myocardium after ligation of coronary artery. The results suggested that Ad-HGF was effectivein vitro andin vivo, and the data for designing human trial of gene therapy—mediated cardiac angiogenesis were provided. |
| Starting Page | 273 |
| Ending Page | 276 |
| Page Count | 4 |
| File Format | |
| ISSN | 10016538 |
| Journal | Chinese Science Bulletin |
| Volume Number | 48 |
| Issue Number | 3 |
| e-ISSN | 18619541 |
| Language | English |
| Publisher | Science in China Press |
| Publisher Date | 2003-01-01 |
| Publisher Place | Beijing |
| Access Restriction | Subscribed |
| Subject Keyword | myocardial ischemia gene therapy recombination adenovirus hepatocyte growth factor Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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