NDLI: Effects of interlinker sequences on the biological properties of bispecific single-chain antibodies

Content Provider	Springer Nature Link
Author	Fang, Min Jiang, Xin Yang, Zhi Yin, Changcheng Li, Hua Zhao, Rui Zhang, Zhong Lin, Qing Huang, Hualiang
Copyright Year	2003
Abstract	Single-chain bispecific antibody (scBsAb) is one of the promising genetic engineering antibody formats for clinical application. But the effects of interlinker sequences on the biological properties of bispecific single-chain antibodies have not been studied in detail. Three interlinker sequences were designed and synthesized, and denominated as Fc, HSA, 205C′, respectively. Universal vectors with these different interlinker sequences for scBsAb expression in E. coli were constructed. A model scBsAb based on a reshaped single-chain antibody (scFv) against human CD3 and a scFv directed against human ovarian carcinoma were generated and expressed in E. coli. The results of SDS-PAGE and Western blot showed that the different interlinker sequences did not affect the expression level of scBsAb. However, as demonstrated by ELISA and pharmacokinetics studies performed in mice, scBsAbs with different interlinker sequences had difference in the antigen-binding activities and terminal half-life time (T $_{1÷2β}$) in vivo, the interlinker HSA could remarkably prolong the retention time of scBsAb in blood. These results indicated that the peptide sequence of interlinker could affect important biological properties of scBsAb, such as antigen-binding properties and stability in vivo. So, selection of an appropriate interlinker sequence is very important for scBsAb construction. Optimal interlinker can bring scBsAb biological properties more suitable for clinical application.
Starting Page	2277
Ending Page	2283
Page Count	7
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	48
Issue Number	21
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2003-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	single-chain antibody bispecific antibody single-chain bispecific antibody interlinker Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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