NDLI: The binding of the anticoagulation factor I from the venom ofAgkistrodon acutus to activated factor X

Content Provider	Springer Nature Link
Author	Xu, Xiaolong Liu, Qingliang Wu, Shuangding
Copyright Year	2000
Abstract	Anticoagulation factor I (ACF I) from the venom ofAgkistrodon acutus prolonged plasma prothrombin time (PPT) with dose-dependent manner and exhibited marked anticoagulant activity only at the concentration higher than its critical concentration (12 nmol/L). It was discovered that ACF I formed a 1:1 complex with activated coagulation factor (FXa) in the presence of Ca$^{2+}$ ions by the method of polyacrylamide gel electrophoresis. Both native ACF I and decalcified ACF I failed to form complexes with FXa in the absence of Ca$^{2+}$. Sr$^{2+}$ ions were able to replace Ca$^{2+}$ ions in the binding of ACF I to FXa, but both Ba$^{2+}$ ions and Tb$^{3+}$ ions were ineffective. ACF I was a new member of the IX/X-bp family in the C-type lectin superfamily, and had a amino acid composition similar to the other members of this family. It was composed of 251 amino acid residues with a molecular weight of 29 603.6 u on non-reducing condition, determined by MALDI-TOF-MS, and a molecular weight of 14.7 ku on reducing condition, determined by the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
Starting Page	1296
Ending Page	1300
Page Count	5
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	45
Issue Number	14
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2000-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	binding protein anticoagulation factor activated coagulant factor X calcium ion Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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0.27-nm resolution crystal structure of Haemorrhagin I from snake venom ofAgkistrodon acutus

Effect of metal ion substitutions in anticoagulation factor I from the venom of Agkistrodon acutus on the binding of activated coagulation factor X and on structural stability

Effect of metal ion substitutions in anticoagulation factor I from the venom of Agkistrodon acutus on the binding of activated coagulation factor X and on structural stability

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Metal ion binding to anticoagulation factor II from the venom of Agkistrodon acutus: stabilization of the structure and regulation of the binding affinity to activated coagulation factor X

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