NDLI: Monitoring the binding of metal cations and histones to DNA in real time using fluorescence assays

Content Provider	Springer Nature Link
Author	Liu, YuYing Wang, PengYe Dou, ShuoXing Zhang, WeiWei Wang, XueJin Sang, HongYi
Copyright Year	2011
Abstract	The binding of cations (Na$^{+}$, K$^{+}$, Mg$^{2+}$, Ca$^{2+}$, Mn$^{2+}$) and histones to DNA can be studied using fluorescence assays. Here, we measured the fluorescence intensity and fluorescence anisotropy of DNA and DNA-histone complexes in the presence of cations. We demonstrate that when different cations are added into a DNA solution, the fluorescence intensities of the stained DNA are reduced by different amounts. Compared with divalent cations, monovalent cations had a weaker effect on fluorescence intensity and fluorescence anisotropy. Divalent (Mn$^{2+}$, Mg$^{2+}$, Ca$^{2+}$) cations markedly enhanced the fluorescence anisotropy of DNA. The binding modes of monovalent and divalent cations to DNA may be different. Divalent cations can change the structure of DNA molecules, or promote the assembly of DNA strands. The addition of histones causes DNA condensation, which mostly occurs during the first few seconds. Cation binding to DNA is abrupt, and is much faster than that of histones.
Starting Page	1080
Ending Page	1085
Page Count	6
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	56
Issue Number	11
e-ISSN	18619541
Language	English
Publisher	SP Science China Press
Publisher Date	2011-04-26
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	DNA histone metal cation fluorescence intensity fluorescence anisotropy Chemistry/Food Science Earth Sciences Physics Life Sciences Engineering Science
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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