NDLI: Detecting CD20-Rituximab interaction forces using AFM single-molecule force spectroscopy

Content Provider	Springer Nature Link
Author	Li, Mi Liu, LianQing Xi, Ning Wang, YueChao Dong, ZaiLi Li, GuangYong Xiao, XiuBin Zhang, WeiJing
Copyright Year	2011
Abstract	The invention of atomic force microscopy (AFM) has provided new technology for measuring specific molecular interaction forces. Using AFM single-molecule force spectroscopy (SMFS) techniques, CD20-Rituximab rupture forces were measured on purified CD20 proteins, Raji cells, and lymphoma patient B cells. Rituximab molecules were linked onto AFM tips using AFM probe functionalization technology, and purified CD20 proteins were attached to mica using substrate functionalization technology. Raji cells (a lymphoma cell line) or lymphoma patient cells were immobilized on a glass substrate via electrostatic adsorption and chemical fixation. The topography of the purified CD20 proteins, Raji cells, and patient lymphoma cells was visualized using AFM imaging and the differences in the rupture forces were analyzed and measured. The results showed that the rupture forces between the CD20 proteins on Raji cells and Rituximab were markedly smaller than those for purified CD20 proteins and CD20 proteins on lymphoma patient B cells. These findings provide an effective experimental method for investigating the mechanisms underlying the variable efficacy of Rituximab.
Starting Page	3829
Ending Page	3835
Page Count	7
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	56
Issue Number	35
e-ISSN	18619541
Language	English
Publisher	SP Science China Press
Publisher Date	2011-12-03
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	atomic force microscopy single-molecule force spectroscopy non-Hodgkin’s lymphoma monoclonal antibody Earth Sciences Science Life Sciences Chemistry/Food Science Physics Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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