NDLI: GCC box inArabidopsis PDF1.2 promoter is an essential and sufficient cis-acting element in response to MeJA treatment

Content Provider	Springer Nature Link
Author	Zhang, Haiwen Xie, Bingyan Lu, Xiangyang Yang, Yuhong Huang, Rongfeng
Copyright Year	2004
Abstract	The expression ofArabidopsis PDF1.2 gene is regulated by jasmonic acid (JA) and ethylene (ET). It also has been well documented that GCC box is an element responsive to ET, however, the responsive mechanism of JA in such plant defense gene expression is unclear. In this paper, the authors define the essential cis-acting element inPDF1.2 promoter responsive to methyl jasmonate (MeJA) through fragment deletions and site-directed mutageneses combiningAgrobacterium-mediated transient reporter gene expression in tobacco leaves. Firstly, the MeJA inducible expression ofPDF1.2 was confirmed by using the upstream −1.86 kb fragment ofPDF1.2 gene. Secondly, the upstream −300–−243 bp fragment of the promoter was evidenced to respond to MeJA. To further characterize this promoter region, three point mutations were introduced into the −300–−243 bp fragment of the promoter. This result showed that the mutation of GCC box abolished MeJA induction, whereas the mutations of the G box-like and the imperfect palindrome sequence did not significantly decrease MeJA inducible effect, indicating that GCC box inPDF1.2 is essential for MeJA induction. The sufficient responsiveness to MeJA of this GCC box was further investigated by 4×GCC fused upstream to the CaMV 35S minimal promoter. This result suggested that the fused promoter was able to activate reporter gene expression in response to MeJA. Thus these results indicate that the GCC box inPDF1.2 is an essential and sufficient element to confer MeJA induction.
Starting Page	2476
Ending Page	2480
Page Count	5
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	49
Issue Number	23
e-ISSN	18619541
Language	English
Publisher	Science in China Press
Publisher Date	2004-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	Agroinfiltation GCC box MeJA PDF1.2 promoter cis-acting element Science Life Sciences Physics Chemistry/Food Science Earth Sciences Engineering
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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