NDLI: Optimization of a synthetic medium for ethanol production by xylose-fermenting Zymomonas mobilis using response surface methodology

Content Provider	Springer Nature Link
Author	Dong, HuiNa Zhao, XueMing Ma, YuanYuan Zhang, MinHua
Copyright Year	2012
Abstract	A synthetic medium for ethanol production by recombinant xylose-fermenting Zymomonas mobilis was optimized using Plackett-Burman (PB) design and response surface methodology (RSM). The effects of 19 medium components were investigated by PB design. Eight of these components were determined to have significant effects on ethanol production. The statistical model was constructed via central composite design (CCD) using five selected variables, including xylose, trisodium citrate, choline chloride, pyridoxine, and thiamine. The validity of the developed model was verified. The ethanol concentration in the optimized medium was 6.7% higher than in the RM medium. The optimized medium was then simplified to give the final synthetic medium (S2), in which the ethanol concentration was 20.7% higher than in the RM medium. Xylose and trisodium citrate were the key medium components influencing ethanol production, while calcium pantothenate was the only growth factor required by recombinant Z. mobilis. Ethanol production and growth response were directly proportional to the logarithm of the concentration of trisodium citrate in the range of 0.1–1.6 g/L.
Starting Page	3782
Ending Page	3789
Page Count	8
File Format	PDF
ISSN	10016538
Journal	Chinese Science Bulletin
Volume Number	57
Issue Number	28-29
e-ISSN	18619541
Language	English
Publisher	SP Science China Press
Publisher Date	2012-08-02
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	recombinant Zymomonas mobilis synthetic medium ethanol production response surface methodology trisodium citrate, xylose Science Engineering Physics Life Sciences Earth Sciences Chemistry/Food Science
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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