NDLI: Photochromic kinetic spectra and intermediates of BR-D96N

Content Provider	Springer Nature Link
Author	Zheng, Yuan Yao, Baoli Wang, Yingli Lei, Ming Chen, Guofu Hampp, rbert
Copyright Year	2003
Abstract	BR-D96N is a kind of genetically site-specific mutant of bacteriorhodopsin (BR) with obvious photochromic effect. Compared to the wild type BR, the lifetime of M state of BR-D96N is prolonged to several minutes so that the photochromic kinetics and the intermediates formation can be studied by the conventional spectra analysis. In the experiment, the absorption spectra of the sample at different time after light illumination are measured with spectrophotometer. By fitting and analyzing the variation of the spectra, we suppose there are three main states in the photochromic process, i.e. B state (light-adapted state), M state and D state (dark-adapted state). The absorption spectra of the B state, M state and D state are extracted from the experimental data based on this three-state model and the spectra at various time are fitted with the least square method. So, the variations of population percentages of the M state, B state and D state are obtained and the M state and B state lifetimes are estimated. In another way, from measuring the absorption dynamics at 407 nm and 568 nm, the M state and B state lifetimes are also obtained by two exponential data fitting, which give coincident results with those of the spectra analysis.
Starting Page	1
Ending Page	7
Page Count	7
File Format	PDF
ISSN	16721799
Journal	Science in China Series G
Volume Number	46
Issue Number	1
e-ISSN	18622844
Language	English
Publisher	Science in China Press
Publisher Date	2003-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	BR-D96N photochromic thermal relaxation absorption spectra Physics Mechanics, Fluids, Thermodynamics Astronomy
Content Type	Text
Resource Type	Article
Subject	Physics and Astronomy

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