Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | Springer Nature Link |
|---|---|
| Author | Chen, Ke Liu, Jun Tong, Guoxiang Liu, Bo Wang, Guodong Liu, Huixia |
| Copyright Year | 2015 |
| Abstract | Obesity is primarily caused by the excessive accumulation of white adipose tissues (WAT). We previously obtained an adipocyte-specific aptamer termed Adipo8 in vitro. In this present study, this adipocyte-specific aptamer Adipo8 was first chemically modified by introduction of phosphorothioate linkages (PS-linkages) and then conjugated to polyethylene glycol (PEG), we tested whether this modified aptamer could distinguish mature white adipocytes from 3T3-L1 preadipocytes or brown adipocytes. To verify the binding affinity of this aptamer to mature white adipocytes in vivo as well as in vitro, we tested whether modified Adipo8 could specifically bind to the WAT of Diet-Induced Obesity (DIO) C57BL/6 mice. Finally, we examined the effect of Adipo8 on the adipogenic differentiation of mature white adipocytes. Based on our results, PS-modified aptamer demonstrated its high binding affinity and specificity, and was able to distinguish white adipocytes from 3T3-L1 preadipocytes or brown adipocytes in vitro. PS-modified Adipo8 also demonstrated more biostability and prolonged binding time in biological fluids. Additionally, Adipo8 could inhibit adipogenic differentiation of adipose tissue, possibly by inhibiting the expression of PPAR-γ in adipose tissue. This modified aptamer holds great promise as a stable molecular recognition tool for targeted delivery to adipocytes and has potential in the treatment of obesity. |
| Starting Page | 1612 |
| Ending Page | 1620 |
| Page Count | 9 |
| File Format | |
| ISSN | 16747291 |
| Journal | Science in China Series B: Chemistry |
| Volume Number | 58 |
| Issue Number | 10 |
| e-ISSN | 18691870 |
| Language | English |
| Publisher | Science China Press |
| Publisher Date | 2015-03-11 |
| Publisher Place | Beijing |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | DNA aptamer adipocyte modification adipogenic PPAR-γ Chemistry/Food Science |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|