NDLI: 5-Hydroxytryptamino-induced calcium sparks in cultured rat stomach fundus smooth muscle cells

Content Provider	Springer Nature Link
Author	Zhang, Xiaoling Yan, Hongtao Yan, Yang
Copyright Year	2003
Abstract	With a new fluorescence probe of Ca$^{2+}$, STDIn-AM, 5-hydroxytryptamino (5-HT)-induced spontaneous calcium release events (calcium sparks) in cultured rat stomach fundus smooth muscle cells (SFSMC) are investigated by laser scanning confocal microscope. The mechanisms of initiation of Ca$^{2+}$ sparks, propagating Ca$^{2+}$ waves and their relation to E-C coupling are discussed. After the extracellular [Ca$^{2+}$] is increased to 10 mmol/L, addition of 5-HT causes hot spots throughout the cytoplasm, which is brighter near the plasmalemma. The amplitude of the event is at least two times greater than the standard deviation of fluorescence intensity fluctuations measured in the neighboring region and the duration of the Ca$^{2+}$ signal is over 100 ms. The results suggest that 5-HT acts by the way of 5-HT$_{2}$ receptors on SFSMC, then through 5-HT$_{2}$ receptors couples IP$_{3}$/Ca$^{2+}$ and DG/PKC double signal transduction pathways to cause Ca$^{2+}$ release from intracellular Ca$^{2+}$ stores and followed Ca$^{2+}$ influx possibly through calcium release-activated calcium influx. The acceptor of activated 5-HT$_{2}$ can also cause membrane depolarization, which then stimulates the L-type Ca$^{2+}$ channels leading to Ca$^{2+}$ influx. Then the local Ca$^{2+}$ entry mentioned above activates ryanodine-sensitive Ca$^{2+}$ release channels (RyR) on sarcoplasmic reticulum (SR) to cause local Ca$^{2+}$ release events (Ca$^{2+}$ sparks) through calcium-induced calcium release (CICR).
Starting Page	13
Ending Page	18
Page Count	6
File Format	PDF
ISSN	10069291
Journal	Science in China Series B: Chemistry
Volume Number	46
Issue Number	1
e-ISSN	18622771
Language	English
Publisher	Science in China Press
Publisher Date	2003-01-01
Publisher Place	Beijing
Access Restriction	Subscribed
Subject Keyword	calcium spark new fluorescence probe of Ca$^{2+}$ stomach fundus smooth muscle cells 5- hydroxytryptamino laser scanning confocal microscope Chemistry/Food Science
Content Type	Text
Resource Type	Article
Subject	Chemistry

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