NDLI: Development and characterization of a microfluidic glucose sensing system based on an enzymatic microreactor and chemiluminescence detection

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Development and characterization of a microfluidic glucose sensing system based on an enzymatic microreactor and chemiluminescence detection

Content Provider	Springer Nature Link
Author	Moon, B. U. Vries, M. G. Westerink, B. H. C. Verpoorte, E.
Copyright Year	2012
Abstract	Chemiluminescence detection was developed as an alternative to amperometric detection for glucose analysis in a portable, microfluidics-based continuous glucose monitoring system. Amperometric detection allows easy determination of hydrogen peroxide, a product of the glucose oxidase-catalyzed reaction of glucose with oxygen, by oxidation at a microelectrode. However, (micro)electrodes in direct contact with physiological sample are subject to electrode fouling, which leads to signal drift, decreased reproducibility and shortened detector lifetimes. Moreover, there are a few species present in the body (e.g. ascorbic acid, uric acid) which can undergo oxidation at the same applied potential as hydrogen peroxide. These species can thus interfere with the glucose measurement, reducing detection specificity. The rationale for exploring chemiluminescence as opposed to amperometric detection is thus to attempt to improve the lifetime and reproducibility of glucose analysis for monitoring purposes, while reducing interference caused by other chemicals in the body. The study reported here represents a first step in this direction, namely the realization of a microfluidic device with integrated silicon photodiode for chemiluminescence detection of glucose. This microflow device uses a chaotic mixing approach to perform enzymatic conversion of glucose, followed by reaction of the hydrogen peroxide produced with luminol to produce light at 425 nm. The chemiluminescence reaction is catalyzed by horseradish peroxidase in the presence of iodophenol. The performance of the fabricated chip was characterized to establish optimal reaction conditions with respect to sample and reagent flow rates, pH, and concentrations. A linear calibration curve was obtained for current response as a function of glucose concentration in the clinically relevant range between 2 and 10 mM, with a sensitivity of 39 pA/mM (R = 0.9963, one device, n = 3) and a limit of detection of 230 μM (S/N = 3).
Starting Page	515
Ending Page	523
Page Count	9
File Format	PDF
ISSN	16747291
Journal	Science in China Series B: Chemistry
Volume Number	55
Issue Number	4
e-ISSN	18691870
Language	English
Publisher	SP Science China Press
Publisher Date	2012-03-08
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	microfluidic chip glucose sensing system enzymatic microreactor chemiluminescence chaotic mixing Chemistry/Food Science
Content Type	Text
Resource Type	Article
Subject	Chemistry

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