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| Content Provider | Springer Nature Link |
|---|---|
| Author | Javić, Barbara S. Mićić Vićovac, Milica Lj. Vujčić, Zoran M. Vujčić, Miroslava T. |
| Copyright Year | 2016 |
| Abstract | Peroxidases (EC 1.11.1.7) have enormous biotechnological applications. Usage of more abundant, basic isoforms of peroxidases in diagnostic kits and/or in immunochemistry has led to under exploitation and disregard of horseradish peroxidase (HRP) acidic isoforms. Therefore, acidic horseradish peroxidase (HRP-A) isoenzyme was used for the preparation of a biocatalyst with improved ability in dye decolorization. Ten biocatalysts were prepared by covalent binding of enzyme to chitosan and alginate, adsorption followed by cross-linking on inorganic support (aluminum oxide), and encapsulation in spherical calcium alginate beads via polyethylene glycol. Model dyes of 50 to 175 mg l$^{−1}$ were removed by the biocatalysts. Among the tested biocatalysts, the three with the highest specific activity and biodegradation rate were further studied (Chitosan-HRP, Al-Gel-HRP and Al-HRP-Gel). The impact of hydrogen peroxide concentration on dye decolorization was examined on the Chitosan-HRP biocatalyst, since the HRP is susceptible to inhibition/inactivation by high H$_{2}$O$_{2}$. On the other hand, H$_{2}$O$_{2}$ is needed as a co-substrate for the HRP, and the H$_{2}$O$_{2}$/dye ratio can greatly influence decolorization efficiency. Concentrations of H$_{2}$O$_{2}$ ranging from 0.22 to 4.4 mM showed no difference in terms of impact on the biocatalyst decolorization efficiency. The high decolorization efficiency of the biocatalysts was validated by the removal of 25 and 100 mg l$^{−1}$ anthraquinone (Remazol Brilliant Blue R (RBBR)), triphenylmethane (Coomassie Brilliant Blue (CBB)), acridine (Acridine Orange (AO)), and formazan metal complex dye (Reactive Blue 52 (RB52)). After the seven consecutive decolorization cycles, the decolorization was still 53, 78, and 67% of the initial dye for the Al-HRP-Gel, Al-Gel-HRP, and Chitosan-HRP immobilizate, respectively. The results obtained showed potential of otherwise neglected acidic HRP isoforms as a cost-effective biocatalyst with significant potential in wastewater dyestuff treatment. |
| Starting Page | 3923 |
| Ending Page | 3933 |
| Page Count | 11 |
| File Format | |
| ISSN | 09441344 |
| Journal | Environmental Science and Pollution Research |
| Volume Number | 24 |
| Issue Number | 4 |
| e-ISSN | 16147499 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2016-12-01 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Horseradish peroxidase Decolorization Reactive dyes Immobilization Isoenzymes Biocatalyst Environment Environmental Chemistry Ecotoxicology Environmental Health Atmospheric Protection/Air Quality Control/Air Pollution Waste Water Technology Water Pollution Control Water Management Aquatic Pollution |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Chemistry Health, Toxicology and Mutagenesis Pollution Medicine |
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